Evidence map›Paper›PMID 39635039›Full record

ArticleNanophotonics (Berlin, Germany)2024

Digital-SMLM for precisely localizing emitters within the diffraction limit.

Zhe Jia, Lingxiao Zhou, Haoyu Li, Jielei Ni, Danni Chen, Dongfei Guo, Bo Cao, Gang Liu, Guotao Liang, Qianwen Zhou and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanophotonics (Berlin, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Zhe JiaNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Lingxiao ZhouNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Haoyu LiNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Jielei NiNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Danni ChenNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Dongfei GuoNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Bo CaoNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Gang LiuNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Guotao LiangNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Qianwen ZhouNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Xiaocong YuanNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.ORCID https://orcid.org/0000-0003-2605-9003
Yanxiang NiNanophotonics Research Center, Institute of Microscale Optoelectronics & State Key Laboratory of Radio Frequency Heterogeneous Integration, College of Physics and Optoelectronic Engineering & Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.ORCID https://orcid.org/0000-0002-0467-9722

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Precisely pinpointing the positions of emitters within the diffraction limit is crucial for quantitative analysis or molecular mechanism investigation in biomedical research but has remained challenging unless exploiting single molecule localization microscopy (SMLM). Via integrating experimental spot dataset with deep learning, we develop a new approach, Digital-SMLM, to accurately predict emitter numbers and positions for sub-diffraction-limit spots with an accuracy of up to 98 % and a root mean square error as low as 14 nm. Digital-SMLM can accurately resolve two emitters at a close distance, e.g. 30 nm. Digital-SMLM outperforms Deep-STORM in predicting emitter numbers and positions for sub-diffraction-limited spots and recovering the ground truth distribution of molecules of interest. We have validated the generalization capability of Digital-SMLM using independent experimental data. Furthermore, Digital-SMLM complements SMLM by providing more accurate event number and precise emitter positions, enabling SMLM to closely approximate the natural state of high-density cellular structures.

Indexed as

deep learningdiffraction limitDigital-SMLMsingle molecule localization microscopysub-diffraction-limited spots

Identifiers

PMID39635039
PMCPMC11465993

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.