Evidence map›Paper›PMID 39635008›Full record

ArticleNanophotonics (Berlin, Germany)2022

Improved localization precision via restricting confined biomolecule stochastic motion in single-molecule localization microscopy.

Jielei Ni, Bo Cao, Gang Niu, Danni Chen, Guotao Liang, Tingying Xia, Heng Li, Chen Xu, Jingyu Wang, Wanlong Zhang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanophotonics (Berlin, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jielei NiNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.ORCID https://orcid.org/0000-0001-8641-7242
Bo CaoNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.
Gang NiuPhil Rivers Technology, Beijing 100871, China.
Danni ChenNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.
Guotao LiangNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.
Tingying XiaNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.
Heng LiTsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, Shenzhen 518055, China.ORCID https://orcid.org/0000-0002-2196-6520
Chen XuNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.
Jingyu WangNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.
Wanlong ZhangNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.
Yilin ZhangNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.
Xiaocong YuanNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.ORCID https://orcid.org/0000-0003-2605-9003
Yanxiang NiNanophotonics Research Center, Shenzhen Key Laboratory of Micro-Scale Optical Information Technology and Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, College of Electronics and Information Engineering, Shenzhen University, Shenzhen 518060, China.ORCID https://orcid.org/0000-0002-0467-9722

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-molecule localization microscopy (SMLM) plays an irreplaceable role in biological studies, in which nanometer-sized biomolecules are hardly to be resolved due to diffraction limit unless being stochastically activated and accurately located by SMLM. For biological samples preimmobilized for SMLM, most biomolecules are cross-linked and constrained at their immobilizing sites but still expected to undergo confined stochastic motion in regard to their nanometer sizes. However, few lines of direct evidence have been reported about the detectability and influence of confined biomolecule stochastic motion on localization precision in SMLM. Here, we access the potential stochastic motion for each immobilized single biomolecule by calculating the displacements between any two of its localizations at different frames during sequential imaging of Alexa Fluor-647-conjugated oligonucleotides. For most molecules, localization displacements are remarkably larger at random frame intervals than at shortest intervals even after sample drift correction, increase with interval times and then saturate, showing that biomolecule stochastic motion is detected and confined around the immobilizing sizes in SMLM. Moreover, localization precision is inversely proportional to confined biomolecule stochastic motion, whereas it can be deteriorated or improved by enlarging the biomolecules or adding a post-crosslinking step, respectively. Consistently, post-crosslinking of cell samples sparsely stained for tubulin proteins results in a better localization precision. Overall, this study reveals that confined stochastic motion of immobilized biomolecules worsens localization precision in SMLM, and improved localization precision can be achieved via restricting such a motion.

Indexed as

confined stochastic motionlocalization precisionsingle-molecule localization microscopy

Identifiers

PMID39635008
PMCPMC11501310

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.