Evidence map›Paper›PMID 39633431›Full record

ArticleProteome science2024

LC-MS-based quantitation of proteomic changes induced by Norcantharidin in MTB-Treated macrophages.

Yi-Lin Wu, Yuan-Ting Li, Gan-Bin Liu, Jin-Lin Wu, Xiao-Ran Liu, Xin-Xuan Gao, Qi-Dan Huang, Jin Liang, Jia-Yi Ouyang, Yi-Ran Ding and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteome science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yi-Lin Wu *Department of Cardiology, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, China.
Yuan-Ting Li *Guangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, School of Medical Technology, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China.
Gan-Bin Liu *Department of Respiration, Dongguan 6th Hospital, Dongguan, China.
Jin-Lin WuGuangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, School of Medical Technology, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China.
Xiao-Ran LiuGuangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, School of Medical Technology, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China.
Xin-Xuan GaoGuangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, School of Medical Technology, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China.
Qi-Dan HuangDepartment of Respiration, Affiliated Houjie Hospital of Guangdong Medical University, Dongguan, China.
Jin LiangDepartment of Respiration, Dongguan 6th Hospital, Dongguan, China.
Jia-Yi OuyangGuangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, School of Medical Technology, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China.
Yi-Ran DingGuangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, School of Medical Technology, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China.
Jun-Yi WuGuangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, School of Medical Technology, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China.
Yuan-Bin LuDepartment of Clinical Laboratory, The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen, China.
Yu-Chi GaoShenzhen Maternity and Child Healthcare Hospital, Shenzhen, Guangdong, China.
Xiao-Zhen CaiDepartment of Respiration, Affiliated Houjie Hospital of Guangdong Medical University, Dongguan, China. imcaixiaozhen@163.com.
Jun-Ai ZhangGuangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, School of Medical Technology, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China. zhangjunai@gdmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tuberculosis drug resistance contributes to the spread of tuberculosis. Immunotherapy is an effective strategy for treating tuberculosis, with the regulation of macrophage-mediated anti-tuberculosis immunity being crucial. Norcantharidin (NCTD), a drug used in tumor immunotherapy, has significant immunomodulatory effects. Thus, NCTD may have an anti-tuberculosis role by regulating immunity. Understanding how NCTD affects the proteome of Mtb-infected macrophages can provide valuable insights into potential treatments. This study aimed to investigate the impact of NCTD (10 μg/mL) on the proteome of macrophages infected with Mtb H37Ra using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. A total of 69 differentially regulated proteins (DRPs) were identified, with 28 up-regulated and 41 down-regulated in the NCTD-treated group. Validation of six DRPs (CLTCL1, VAV1, SP1, TRIM24, MYO1G, and WDR70) by Western blot analysis confirmed the accuracy of the LC-MS/MS method used in this study. NCTD modulates various protein expressions involved in chromatin-modifying enzymes, RHO GTPases activating PAKs, Fc gamma R-mediated phagocytosis, T cell receptor signaling pathway, and antigen processing and presentation. Overall, the research provides new insights into the effects of NCTD on the proteome of Mtb-infected macrophages. The identified changes highlight potential targets for future therapeutic interventions aimed at enhancing host immunity against Mtb infection or developing new anti-TB drugs based on these findings.

Indexed as

H37RaMacrophageMycobacterium tuberculosisNorcantharidinProteome

Identifiers

PMID39633431
PMCPMC11619108

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.