Enzymatic Synthesis of a Polyketide/Nonribosomal Peptide Hybrid Antibiotic, Salivabactin.

Di Gu, Rui Zhai, Bailey Daymo, Yuxin Xie, Caroline Luo, Wenjun Zhang

Abstract read

In one paragraph

Article in Biochemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

EngineeringACS sustainable chemistry & engineering · 2025
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Di GuDepartment of Chemistry, University of California, Berkeley, California 94720, United States.ORCID 0000-0003-4860-7701

Rui ZhaiDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720, United States.ORCID 0000-0001-5032-9689

Bailey DaymoDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720, United States.

Yuxin XieDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720, United States.

Caroline LuoDepartment of Chemistry, University of California, Berkeley, California 94720, United States.

Wenjun ZhangDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720, United States.ORCID 0000-0001-9348-972X

Funding

Bioactive natural product discovery from human oral microbesR01DE032732 · NIDCR · UNIVERSITY OF CALIFORNIA BERKELEY · PI Wenjun Zhang · 2024 to 2026

$1.1M

NIDCR NIH HHS R01 DE032732

6 · The paper itself

Abstract

Salivabactin is a newly identified polyketide/nonribosomal peptide (PK/NRP) from a human oral probiotic, possessing a unique chemical structure and outstanding antibiotic activities. Although the biosynthetic gene cluster for salivabactin is known, the enzymatic logic that governs the synthesis of salivabactin has not yet been fully studied. In this work, we dissected the biosynthetic pathway for salivabactin using biochemical analysis. We successfully reconstituted the enzymatic synthesis of salivabactin in vitro, identified the minimal set of enzymes required for its biosynthesis, and revealed an unusual thioesterase domain involved in terminal olefin formation.

Indexed as

Anti-Bacterial AgentsPolyketidesBacterial ProteinsBiosynthetic PathwaysHumansMultigene FamilyPeptidesPolyketide SynthasesProbioticsAnti-Bacterial AgentsBacterial ProteinsPeptidesPolyketidesPolyketide Synthases

Identifiers

PMID39630928

DOI10.1021/acs.biochem.4c00515

PMCPMC11652223

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.