Evidence map›Paper›PMID 39630239›Full record

ArticleApplied microbiology and biotechnology2024

Hyperthermophilic xylanase and thermophilicity analysis by molecular dynamic simulation with quantum mechanics.

Katewadee Boonyapakron, Braden Keiser, Kanoknart Prabmark, Katesuda Aiewviriyasakul, Nattapol Arunrattanamook, Aritsara Jaruwat, Penchit Chitnumsub, Jia-Yi Li, Tuck Seng Wong, Xin-Qing Zhao and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Katewadee BoonyapakronBiorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Road, Khlong Luang, 12120, Patumthani, Thailand.
Braden KeiserState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
Kanoknart PrabmarkBiorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Road, Khlong Luang, 12120, Patumthani, Thailand.
Katesuda AiewviriyasakulBiorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Road, Khlong Luang, 12120, Patumthani, Thailand.
Nattapol ArunrattanamookBiorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Road, Khlong Luang, 12120, Patumthani, Thailand.
Aritsara JaruwatBiomolecular Analysis and Application Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Road, Khlong Luang, Patumthani, 12120, Thailand.
Penchit ChitnumsubBiomolecular Analysis and Application Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Road, Khlong Luang, Patumthani, 12120, Thailand.
Jia-Yi LiState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
Tuck Seng WongSchool of Chemical, Materials and Biological Engineering, University of Sheffield, Sir Robert Hadfield Building, Mappin Street, Sheffield, S1 3JD, UK.
Xin-Qing ZhaoState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
Chen-Guang LiuState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
Dong-Qing WeiState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China. dqwei@sjtu.edu.cn.
Verawat ChampredaBiorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Road, Khlong Luang, 12120, Patumthani, Thailand. verawat@biotec.or.th.ORCID http://orcid.org/0000-0001-7768-1340

Funding

Key Technologies Research and Development Program of Anhui Province 2023YFE0199200National Science Foundation of China 32030063National Science Foundation of China 61832019National Science Foundation of China Grant No. 32070662PMU-B B16F640052Royal Academy of Engineering LTSRF1819\15\21
6 · The paper itself

Abstract

Thermophilic xylanases catalyzing the cleavage of β-1,4-glycosidic bonds in xylan have applications in food, feed, biorefinery, and pulp industries. In this study, a hyperthermophilic endo-xylanase was obtained by further enhancement of thermal tolerance of a thermophilic GH11 xylanase originated from metagenome of bagasse pile based on rational design. Introducing N13F and Q34L to the previously reported X11P enzyme shifted the optimal working temperature to 85 °C and led to 20.7-fold improvement in thermostability at 90 °C along with a marked increase in T

Indexed as

Endo-1,4-beta XylanasesEnzyme StabilityGlucuronatesMolecular Dynamics SimulationOligosaccharidesXylansCelluloseDisaccharidesHot TemperatureHydrogen BondingMetagenomeQuantum TheorySubstrate SpecificitybagasseCelluloseDisaccharidesEndo-1,4-beta XylanasesGlucuronatesOligosaccharidesXylansxylobiosexylooligosaccharideHyperthermophilicityMolecular dynamic simulationOligosaccharidesRational designXylanase

Identifiers

PMID39630239
PMCPMC11618197

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.