Evidence map›Paper›PMID 39629114›Full record

ArticleEngineering microbiology2024

Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines.

Maofeng Wang, Cancan Wu, Nan Liu, Xiaoqiong Jiang, Hongjie Dong, Shubao Zhao, Chaonan Li, Sujuan Xu, Lichuan Gu

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Engineering microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Maofeng WangState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Cancan WuState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Nan LiuDepartment of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China.
Xiaoqiong JiangState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Hongjie DongShandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences, Jining 272033, China.
Shubao ZhaoState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Chaonan LiState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Sujuan XuState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
Lichuan GuState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The coronavirus disease 2019 (COVID-19) pandemic has highlighted the importance of developing novel vaccines. An ideal vaccine should trigger an intense immune reaction without causing significant side effects. In this study we found that substitution of tryptophan located in the cores of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) protein structures with certain smaller amino acids resulted in variants with melting temperatures of 33-37 °C. An enzyme activity assay indicated that the proteolytic activity of the main proteinase (3CL

Indexed as

3CLproEnzyme activityProtein melting temperatureSARS-CoV-2 N proteinTemperature sensitive attenuated vaccines

Identifiers

PMID39629114
PMCPMC11610959

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.