ArticleBMC microbiology2024
Protective effect of phage pSal-4 on chicken intestinal epithelial cells injured by Salmonella enteritidis.
Article in BMC microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Strong mucosal adhesion enhances the anti-infective effectiveness of T4-like phages.Applied and environmental microbiology · 2026Article
- Phage Therapy in Gastrointestinal Diseases: Current Status and Challenges.International journal of molecular sciences · 2026Review
- Application of bacteriophages in the prevention and control of bacterial infectious diseases in animals.Frontiers in microbiology · 2026Review
- Evaluation of the therapeutic effect of Berberine microcapsules on Salmonella enteritidis-infected mice.BMC veterinary research · 2025Article
- Inflammation, toxicity, and apoptosis reducing potential of bacteriophage Ariobarzanes on intestinal cells infected with Salmonella Typhimurium.Scientific reports · 2025Article
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Authors and funding
9 authors.
Funding
Abstract
Salmonella enteritidis plays a significant role in zoonotic infections. This pathogen settles in the intestinal tract of poultry and contaminates meat and egg products during production for slaughter. Consequently, it can also be transmitted to humans along the food chain, posing a significant risk to public health. Bacteriophages offer a viable substitute for antibiotics in treating Salmonella enteritidis due to their specific bactericidal effect and safety. This study aimed to investigate the protective effect of bacteriophage against Salmonella enteritidis infection in chicken intestinal epithelial cells. Chicken intestinal epithelial cells were treated with various concentrations of bacterial suspension, and the impact on cell damage was assessed by measuring changes in cell viability and observing structural changes via transmission electron microscopy.In the phage protection experiment, the phages were co-incubated with Salmonella enteritidis for 2, 4, and 6 h. Thereafter, the adhesion rate and invasion rate of bacteria and gene transcription levels of Occludin, Claudin-1, ZO-1, NF-κB p65, TNF-α and IL-6 in cells were determined. The results indicated that the phage could significantly reduce both adhesion and invasion rates of Salmonella enteritidis at MOI = 10 (P < 0.05). Following phage treatment of the co-culture of chicken intestinal epithelial cells and Salmonella enteritidis (CICC10467), there was a significant reduction in the gene expression of pro-inflammatory cytokines NF-κB p65, TNF-α and IL-6, and a significant increase in the gene expression of anti-inflammatory cytokines Occludin, Claudin-1, and ZO-1. This indicates that the phage can inhibit the infection of chicken small intestinal epithelial cells by Salmonella enteritidis. Furthermore, phages were able to significantly alleviate inflammation and barrier integrity disruption caused by the bacteria in the co-culture. These observations suggest that phages are promising candidates for preventing and treating gastrointestinal bacterial infections.
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