Evidence map›Paper›PMID 39627267›Full record

ArticleScientific reports2024

Multispectroscopic and computational insights into amyloid fibril formation of alpha lactalbumin induced by sodium hexametaphosphate.

Nasser Abdulatif Al-Shabib, Javed Masood Khan, Ajamaluddin Malik, Md Tabish Rehman, Abdulaziz Alamri, Vijay Kumar, Per Erik Joakim Saris, Fohad Mabood Husain, Mohamed F AlAjmi

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Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Nasser Abdulatif Al-ShabibDepartment of Food Science and Nutrition, Faculty of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia. nalshabib@ksu.edu.sa.
Javed Masood KhanDepartment of Food Science and Nutrition, Faculty of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia. jmkhan@ksu.edu.sa.
Ajamaluddin MalikDepartment of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Md Tabish RehmanDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Abdulaziz AlamriDepartment of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Vijay KumarHimalayan School of Biosciences, Swami Rama Himalayan University, Jolly Grant, Dehradun , Uttarakhand, 248016, India.
Per Erik Joakim SarisDepartment of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Finland. per.saris@helsinki.fi.
Fohad Mabood HusainDepartment of Food Science and Nutrition, Faculty of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia.
Mohamed F AlAjmiDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

Funding

Deanship of Scientific Research, King Saud University RSPD2024R923
6 · The paper itself

Abstract

The impact of sodium hexametaphosphate (SHMP) on the aggregation behavior of α-lactalbumin (α-LA) was studied at pH 7.4 and 2.0. Turbidity measurements showed a concentration-dependent aggregation of α-LA at pH 2.0 in the presence of SHMP, while no aggregation was observed at pH 7.4. Light scattering (LS) and Thioflavin-T (ThT) data revealed that the aggregation was rapid, following nucleation-independent pathways. In other kinetics experiments such as turbidity and ThT confirmed that SHMP-induced α-LA aggregation was dependent on SHMP concentration rather than incubation time. Once formed, the aggregates remained unchanged for up to five days. Intrinsic fluorescence studies indicated conformational changes in α-LA upon SHMP addition, and dye-binding assays with ThT and Congo Red demonstrated the formation of amyloid-like aggregates. Far-UV circular dichroism (CD) data suggested a structural transition from α-helical to β-structures in α-LA in the presence of SHMP at pH 2.0. Molecular docking studies confirmed stronger interactions between α-LA and SHMP at pH 2.0 (ΔG = -6.2 kcal/mol) compared to pH 7.4 (ΔG = -5.3 kcal/mol), driven by electrostatic forces and hydrogen bonding. These results suggest that SHMP induces amyloid-like aggregation of α-LA, particularly at acidic pH.

Indexed as

AmyloidLactalbuminMolecular Docking SimulationPhosphatesCircular DichroismHydrogen-Ion ConcentrationKineticsProtein AggregatesAmyloidLactalbuminPhosphatesProtein Aggregatessodium polymetaphosphateAlpha-lactalbuminAmyloid fibrilProtein conformationSodium hexametaphosphateStabilityThT

Identifiers

PMID39627267
PMCPMC11615314

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.