ArticleExperimental and therapeutic medicine2025
MicroRNA‑4327 regulates TGF‑β1 stimulation of matrix metalloproteinase‑13 expression via CREB‑binding protein‑mediated Runx2 acetylation in human osteoblasts.
Article in Experimental and therapeutic medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Role of Non-Coding RNAs in Pathogenesis, Diagnosis, and Therapy of Legg-Calvé-Perthes Disease: A Systematic Review.Medical science monitor : international medical journal of experimental and clinical research · 2025Pooled it
- Protein acylation and bone metabolism: bidirectional crosstalk linking metabolic reprogramming to skeletal homeostasis.Journal of molecular histology · 2026Review
- Integrated Multi-Omics Analysis and Experimental Validation Identify Acetylation-Related Genes as Potential Regulators in Osteoarthritis.Biomedicines · 2026Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transforming growth factor beta 1 (TGF-β1), a multifunctional cytokine, induces the expression of bone remodeling gene matrix metalloproteinase-13 (MMP-13). CREB-binding protein (CBP), a co-activator and runt-related transcription factor 2 (Runx2), a bone transcription factor, play critical roles in regulating bone-remodeling genes. Recent advances in non-coding RNAs have revealed the significance of microRNAs (miRNAs) and their target genes in bone physiology. The present study hypothesized that TGF-β1 stimulated MMP-13 expression by downregulating CBP-targeting miRNAs and activating CBP-mediated Runx2 acetylation in human osteoblastic cells. TGF-β1-downregulated miRNAs that potentially target CBP were identified. Among these miRNAs, TGF-β1 significantly downregulated miR-4327 in these cells. TGF-β1 stimulated CBP, acetylated Runx2 and MMP-13 protein expression levels in human osteoblastic cells and this effect was decreased by overexpressing miR-4327 in these cells. In human osteoblastic cells, miR-4327 was found to directly bind to the 3'-untranslated region of CBP using a dual-luciferase gene reporter assay. Thus, the present study indicated that the TGF-β1/miR-4327/CBP/Runx2 plays a key role in MMP-13 expression, suggesting the clinical relevance of this axis for treating bone-related disorders.
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