ArticleIranian journal of veterinary research2024
Toxinotyping of
Article in Iranian journal of veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Necrotic enteritis (NE) is an economically important disease, caused by Aims: This study aimed to genotypically characterize virulent strains of Methods: Eleven isolates were derived from necrotic enteritis cases, and 27 were from healthy chickens. Isolations were performed using blood agar. To assess whether zmp is generally associated with avian NE, 38 Results: All isolates carried the phospholipase c (plc) gene regardless of their origin and virulence. The zinc metallopeptidase (zmp) gene was found in the isolates collected from birds affected by necrotic enteritis. Furthermore, Necrotic enteritis like B (NetB) was only found in 36.36% of the isolates derived from necrotic enteritis-infected birds. Western blot analysis further confirmed the expression of Alpha toxin, NetB, and Zmp in different isolates. Incubation of Leghorn Male Hepatoma (LMH) cells with crude Conclusion: It seems that both NetB and Zmp play major roles in the cytotoxicity and pathogenicity of this organism.
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