ArticleMethodsX2024
Validation of reference genes for cardiac RT-qPCR studies spanning the fetal to adult period.
Article in MethodsX, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- A Multi-Tissue Transcriptomic Atlas of River Buffalo with a Focus on the Genetic Underpinnings of Lactation Performance Across Four Lactation Stages in the Mammary Gland.International journal of molecular sciences · 2026Article
- Development of striated muscle microvasculature across the perinatal period in lambs.The Journal of physiology · 2026Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Many genes used as internal controls for mRNA expression studies are unstable (change) over development. This study determined an approach to validate reference genes for mRNA studies spanning the fetal period to adulthood in sheep hearts.•We determined the mRNA expression of 12 candidate reference genes (ACTB, GAPDH, H3-3A, HYAL2, PPIA, RNA18S1, RPL32, RPL37A, RPL41, RPLP0, RPS15, and YWHAZ) via RT-qPCR. Per RefFinder, which incorporates computational algorithms by BestKeeper, comparative delta Ct, GeNorm, and NormFinder, RPL32, RPL37A, HYAL2, ACTB and GAPDH were the most stable reference genes, although none were unchanged across all ages.•Systematical calculation of the geometric means of 3 reference genes revealed the combination of HYAL2, RPL32, and RPL37A was unchanged across the 5 fetal, neonatal, and adult ages.•We determined the most stable combination of reference genes for cardiac gene expression studies in sheep from fetus to newborn to adult; these steps are applicable to determine internal controls for mRNA studies in other organs, other species, and periods in which reference gene instability is high.
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Registered trials
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