ArticleMethods in molecular biology (Clifton, N.J.)2025
Investigating the Impact of Host Factors on Vaccinia Virus Infection Through Single-Cell Analysis via Flow Cytometry.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Type II tRNA cleavage by SLFN14 endoribonuclease variants linked to inherited thrombocytopenia drives global translational repression.PLoS biology · 2026Article
- Structural and functional studies of rabbit SAMD9 reveal a distinct tRNase module that underlies the antiviral activity.PLoS pathogens · 2025Article
- Structural and Functional Studies of Rabbit SAMD9 Reveal a Distinct tRNase Module That Underlies the Antiviral Activity.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
To investigate the impact of a host factor on viral replication, conventional techniques involve analyzing viral replication within a homogeneous cell population engineered to uniformly express the host factor. In this chapter, we present a method to explore the intricate dynamics between host factors and vaccinia virus (VACV) infection using flow cytometry, which enables the rapid, multi-parametric analysis of individual cells in solution. Cultured cells are transfected to express a host factor fused with a fluorescent protein, followed by infection with a VACV encoding a different fluorescent reporter. Subsequently, cells are washed, fixed, and filtered for flow cytometry analysis. The data are then analyzed with flow cytometry analysis software to evaluate the influence of the host factor on various infection parameters, including the stage of viral infection and viral replication level. This method allows for the precise investigation of proviral or antiviral factor expression in relation to viral replication within a heterogeneous population of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.