Evidence map›Paper›PMID 39621268›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Bioluminescence Imaging to Study Recombinant Orthopoxvirus Infection in Animal Models.

Chantal Kling, Todd G Smith, Nicolle Baird, Christina L Hutson

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chantal KlingPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA. ckling@cdc.gov.
Todd G SmithPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Nicolle BairdPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Christina L HutsonPoxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bioluminescent images of viral replication in live animals (in vivo) reveal disease dynamics and effects of medical countermeasures over time. After selecting an appropriate orthopoxvirus animal model for the study, a recombinant virus with the firefly luciferase gene inserted in the genome is used to infect the animals. On the day of bioluminescent imaging, the substrate, D-luciferin, is prepared; animals are sedated and injected with the substrate and IVIS imager is utilized; various bioluminescent images are acquired; then animals recover and are able to continue in the study. Ex vivo imaging can also be completed after animals are euthanized at experimental endpoint. This approach allows real-time imaging of viral kinetics within an animal, and analysis of images can provide an additional quantitative measure throughout the study. Bioluminescent imaging not only provides scientific benefits but also benefits to animal welfare. For these reasons, bioluminescent imaging should be considered for any in vivo orthopoxvirus study.

Indexed as

Disease Models, AnimalLuminescent MeasurementsOrthopoxvirusPoxviridae InfectionsAnimalsBenzothiazolesGenes, ReporterLuciferases, FireflyMiceVirus ReplicationBenzothiazolesD-luciferinLuciferases, FireflyBioluminescentMonkeypox virusMousePrairie dogVaccinia virus

Identifiers

PMID39621268

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.