Evidence map›Paper›PMID 39621099›Full record

ArticleHistochemistry and cell biology2024

A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging.

Xiwei Fan, Bogdan Donose, Michael W M Jones, Daryl Howard, Jari Torniainen, Karl Bertling, Xiao Guo, Cameron M Kewish, Kah Meng Lee, Antonia Rujia Sun and 4 more

Abstract read
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Article in Histochemistry and cell biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xiwei FanDepartment of Orthopaedic Surgery, The Second Xiangya Hospital of Central South University, Changsha, China.
Bogdan DonoseSchool of Electrical Engineering and Computer Science, Faculty of Engineering, Architecture and Information Technology, University of Queensland, Brisbane, Australia.
Michael W M JonesCentral Analytical Research Facility, Queensland University of Technology, Brisbane, Australia.
Daryl HowardAustralian Nuclear Science and Technology Organisation, Australian Synchrotron, Melbourne, Australia.
Jari TorniainenSchool of Electrical Engineering and Computer Science, Faculty of Engineering, Architecture and Information Technology, University of Queensland, Brisbane, Australia.
Karl BertlingSchool of Electrical Engineering and Computer Science, Faculty of Engineering, Architecture and Information Technology, University of Queensland, Brisbane, Australia.
Xiao GuoSchool of Electrical Engineering and Computer Science, Faculty of Engineering, Architecture and Information Technology, University of Queensland, Brisbane, Australia.
Cameron M KewishAustralian Nuclear Science and Technology Organisation, Australian Synchrotron, Melbourne, Australia.
Kah Meng LeeCentral Analytical Research Facility, Queensland University of Technology, Brisbane, Australia.
Antonia Rujia SunSchool of Mechanical, Medical & Process Engineering, Queensland University of Technology, 60 Musk Ave/Cnr. Blamey St, Kelvin Grove, Brisbane, QLD, 4059, Australia.
Aleksandar RakicSchool of Electrical Engineering and Computer Science, Faculty of Engineering, Architecture and Information Technology, University of Queensland, Brisbane, Australia.
Ross CrawfordSchool of Mechanical, Medical & Process Engineering, Queensland University of Technology, 60 Musk Ave/Cnr. Blamey St, Kelvin Grove, Brisbane, QLD, 4059, Australia.
Isaac O Afara *School of Electrical Engineering and Computer Science, Faculty of Engineering, Architecture and Information Technology, University of Queensland, Brisbane, Australia. isaac.afara@uef.fi.
Indira Prasadam *School of Mechanical, Medical & Process Engineering, Queensland University of Technology, 60 Musk Ave/Cnr. Blamey St, Kelvin Grove, Brisbane, QLD, 4059, Australia. i.prasadam@qut.edu.au.

Funding

Academy of Finland 315820Australian Research Council DP210103342CBT Early Career Researchers (ECR) Scheme 324910-0028/07Jane and Aatos Erkko Foundation 190001National Health and Medical Research Council APP1176298Science and Technology Innovation Program of Hunan Province 2024RC3053
6 · The paper itself

Abstract

Understanding the osteochondral junction, where non-mineralised cartilage and mineralised bone converge, is crucial for joint health. Current sample preparation techniques are insufficient for detailed spatial hyperspectral imaging analysis. Using the enhanced Kawamoto method, we used the super cryo embedding medium's temperature-dependent properties to transfer high-quality tissue samples onto slides for spatial imaging analysis. We transferred osteochondral samples using a tape-free system and successfully tested them in hematoxylin and eosin (HE), Safranin-O, nanomechanical assessments and nano-Fourier transform infrared (FTIR) mapping. This protocol elucidates the structural and elemental gradients, mechanical characteristics and distinctive biochemical layering, making it a useful tool for analysing biochemical properties' co-distribution in healthy and diseased situations.

Indexed as

Hyperspectral ImagingCartilageCryoultramicrotomyHigh-Throughput Screening AssaysHumansSpectroscopy, Fourier Transform InfraredCryosectioningMolecular imagingNano-mechanicsOsteochondral unitTrace elementX-ray ptychography

Identifiers

PMID39621099

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.