ArticleFood science & nutrition2024
Novel peptides with calcium-binding capacity from antler bone hydrolysate, its bioactivity on MC3T3-E1 cells, and the possible chelating mode.
Article in Food science & nutrition, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Experimental study on antler powder/chitosan/β-glycerophosphate/polyvinyl alcohol composite platelet-rich fibrin scaffold for repairing periodontal bone defects in rabbits.Frontiers in bioengineering and biotechnology · 2026Article
- Free amino acid-rich egg yolk protein hydrolysate promotes osteogenesis of MC3T3-E1 cells association withFrontiers in nutrition · 2026Article
- Novel peptides with calcium-binding capacity from antler bone hydrolysate, its bioactivity on MC3T3-E1 cells, and the possible chelating mode.Food science & nutrition · 2024Article
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8 authors.
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Abstract
In this study, peptide-calcium chelate was screened from antler bone hydrolysate, and its bioactivity on MC3T3-E1 cells and its chelating mechanism were investigated. In vitro experiments showed that peptide-calcium chelate promoted the differentiation and mineralization of MC3T3-E1 cells. Subsequently, three novel calcium-chelating peptides were obtained from antler bone hydrolysate using hydroxyapatite chromatography (HAC), Sephadex G-25 gel filtration chromatography, and reversed-phase high-performance liquid chromatography (RP-HPLC). Meanwhile, this work determined peptides' amino acid sequences as TKLGTQLQL, LETVILGLLKT, and KMVFLMDLLK based on LC-MS/MS. Then the present work prepared the three peptides, with the corresponding calcium-chelating rates being verified as 87.68 ± 2.86%, 80.72 ± 0.93%, and 67.96 ± 0.98%, respectively. Fourier transform infrared (FTIR) spectroscopy, ultraviolet-visible absorption (UV-vis) spectroscopy, X-ray diffraction (XRD), circular dichroism (CD), zeta potential, and molecular dynamics (MD) simulations were adopted to investigate the chelating mode of peptides with calcium ions. As a result, oxygen in the carboxyl group and the nitrogen in the amino group were related to calcium binding. In addition, the chelation site preferred the negatively charged carboxylate groups of Leu or Thr. The present work revealed that antler bone might be the new calcium-chelating peptide source and elucidated their positive role in osteogenesis.
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