Evidence map›Paper›PMID 39619638›Full record

ArticleMaterials today. Bio2024

Extracellular vesicles from human adipose-derived stem cell spheroids: Characterization and therapeutic implications in diabetic wound healing.

Edgar Daniel Quiñones, Mu-Hui Wang, Kuan-Ting Liu, Ting-Yu Lu, Guan-Yu Lan, Yu-Ting Lin, Yu-Liang Chen, Tang-Long Shen, Pei-Hsun Wu, Yu-Sheng Hsiao and 3 more

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Article in Materials today. Bio, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Edgar Daniel QuiñonesTaiwan International Graduate Program, Sustainable Chemical Science & Technology, Academia Sinica, Institute of Chemistry, Taipei 115, Taiwan.
Mu-Hui WangDepartment of Surgery, National Taiwan University Hospital and College of Medicine, Taipei 100, Taiwan.
Kuan-Ting LiuDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Ting-Yu LuProgram in Materials Science and Engineering, University of California San Diego, CA, 92093, USA.
Guan-Yu LanDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Yu-Ting LinDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Yu-Liang ChenDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Tang-Long ShenDepartment of Plant Pathology & Microbiology, National Taiwan University, Taiwan.
Pei-Hsun WuJohns Hopkins Institute for NanoBioTechnology, John Hopkins University Baltimore, MD 21218, USA.
Yu-Sheng HsiaoDepartment of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
Er-Yuan ChuangSchool of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
Jiashing YuTaiwan International Graduate Program, Sustainable Chemical Science & Technology, Academia Sinica, Institute of Chemistry, Taipei 115, Taiwan.
Nai-Chen ChengDepartment of Surgery, National Taiwan University Hospital and College of Medicine, Taipei 100, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The management of diabetic wounds presents a considerable challenge within the realm of clinical practice. Cellular-derived nanoparticles, or extracellular vesicles (EV), generated by human adipose-derived stem cells (hASCs) have been investigated as promising candidates for the treatment of diabetic wounds. Nevertheless, limitations on the yield, as well as the qualitative angiogenic properties of the EV produced, have been a persistent issue. In this study, a novel approach involving the use of various cell culture morphologies, such as cell spheroids, on hASC was used to promote both EV yield and qualitative angiogenic properties for clinical use, with an emphasis on the in vivo angiogenic properties exhibited by the EV. Moreover, an increase in the secretion of the EV was confirmed after cell spheroid culture. Furthermore, microRNA(miRNA) analysis of the produced EVs indicated an increase in the presence of wound healing-associated miRNAs on the cell spheroid EV. Analysis of the effectiveness of the treated EVs in vitro indicated a significant promotion of the biological function of fibroblast and endothelial cells, cell migration, and cell proliferation post-cell spheroid EV application. Meanwhile, in vivo experiments on diabetic rats indicated a significant increase in collagen production, re-epithelization, and angiogenesis of the diabetic wound after EV administration. In this investigation, we posit that the use of cell spheroids for the culture of hASC represents a novel approach to enhance the substantial secretion of extracellular vesicles while increasing the angiogenic wound healing properties. This innovation holds promise for augmenting the therapeutic potential of EVs in diabetic wound healing, aligning with the exigencies of clinical applications for these nanoparticles.

Indexed as

Adipose-derived stem cell (ASC)Cell sheetCell spheroidsDiabetes wound healingExtracellular vesicles

Identifiers

PMID39619638
PMCPMC11605404

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.