ArticleACS omega2024
An Upconversion Fluorescent Resonant Energy Transfer Biosensor for the Detection of microRNA through DNA Hybridization.
Article in ACS omega, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- Enhancing Electrochemiluminescence with Two-Component Spontaneously Adsorbed Monolayers and Films.ACS omega · 2026Article
- Surface bioengineering of lanthanide nanoparticles for theranostic applications: From hydrophilic modification to multimodal imaging and therapy.Materials today. Bio · 2026Review
- Biomolecule Detection Methods Based on Nanoparticle Approaches.ACS omega · 2026Review
- Review
- Fluorescence Based Trace Level Detection of Cobalt Using 2,6-bis((E)-4-chlorobenzylidene)-4-methylcyclohexan-1-one as Sensor.Journal of fluorescence · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs), a class of small molecules with important regulatory functions, have been widely used in biosensing as biomarkers for the early diagnosis of various diseases (such as osteoporosis). Therefore, it is necessary to develop a miRNA detection platform with high sensitivity and specificity. Herein, an upconversion biosensor based on fluorescent resonant energy transfer (FRET) for the detection of miRNAs was developed. Gold nanoparticles (AuNPs, fluorescence acceptors) were conjugated with aptamers, while upconversion nanoparticles (UCNPs, fluorescence donors) were functionalized with the corresponding complementary DNA (cDNA). The upconversion luminescence (UCL) is quenched vis FRET due to the good overlap between UCNP fluorescence emission and AuNP absorption. In the presence of the target miRNA, the aptamers preferentially bind to the miRNA, thereby dissociating the UCNP-cDNA from the AuNP-aptamers, resulting in the recovery of the UCL. The UCL recovery efficiency increased in a miRNA concentration-dependent manner, which supported the principle of miRNA quantification. The UCNP-based FRET biosensor exhibited sensitive detection of miRNA-21 (as a model analyte) with low limit of detection of 5.91 pM. In addition, depending on the near-infrared excitation and a simple complementary binding reaction during the detection process, the designed biosensor not only effectively eliminated the autofluorescence but also reduced the detection time.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.