Evidence map›Paper›PMID 39616566›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Chromosome Counting at Meiosis and Mitosis of Mouse Oocytes and Embryos Using Super-Resolution Live-Cell Imaging and CRISPR/dCas9-Mediated Live-FISH.

Yu Hatano, Nao Yonezawa, Mikiko Tokoro, Tatsuma Yao, Kazuo Yamagata

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. T4 DNA-Induced Reconstruction of Artificial Nuclei in Living Mouse Oocytes.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yu HatanoFaculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.
Nao YonezawaFaculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.
Mikiko TokoroFaculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.
Tatsuma YaoFaculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan.
Kazuo YamagataFaculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan. yamagata@waka.kindai.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Live cell imaging techniques are now essential for capturing chromosomal segregation in fertilized eggs. Although better spatiotemporal resolution of fluorescence observations could provide more information, higher phototoxicity may occur. Super-resolution microscopy is generally considered unsuitable for live cell imaging because of the considerable cell damage. Here, we developed a method for counting chromosomes in mouse living oocytes and early embryos using super-resolution microscopy based on disk confocal photon reassignment microscopy (OPRA). In this chapter, we describe the imaging conditions for minimally invasive, high-resolution observation of mouse oocytes and embryos and a method to count chromosomes via CRISPR/dCas-mediated live-cell fluorescence in situ hybridization.

Indexed as

CRISPR-Cas SystemsIn Situ Hybridization, FluorescenceMeiosisMitosisOocytesAnimalsChromosome SegregationChromosomes, MammalianEmbryo, MammalianFemaleMiceMicroscopy, ConfocalChromosome countingLive-cell imagingLive-FISHMouse embryoSuper-resolution

Identifiers

PMID39616566

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.