ArticleMethods in molecular biology (Clifton, N.J.)2025
Super-Resolution Imaging of Mitotic Spindle Microtubules Using STED Microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Polar chromosomes are rescued from missegregation by spindle elongation-driven microtubule pivoting.Nature communications · 2026Article
- Kinetochore-centrosome feedback linking CENP-E and Aurora kinases controls chromosome congression.Nature communications · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stimulated emission depletion (STED) microscopy is a powerful super-resolution imaging technique that only recently entered the field of mitosis, where it proved to be invaluable for studying various microtubule classes, kinetochore-microtubule attachments and chromosome segregation errors. Here, we describe immunofluorescence combined with STED microscopy as a method for analyzing microtubules and kinetochore-microtubule attachments in human mitotic spindles. We also describe live-cell STED microscopy as a method for single-plane short-term imaging of transient processes in crowded spindle areas. Finally, we outline image analysis approaches for the quantitative assessment of microtubule bundles within the spindle.
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Registered trials
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