ArticleNature communications2024
Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Periplasmic SacB as a robust counter-selection tool for genome engineering in the polyploid bacteriumMicrobiology spectrum · 2026Article
- Engineering ethanologenic bacteriumSynthetic and systems biotechnology · 2026Article
- Using auxotrophic donor strains to explore pQBR57 plasmid host range among environmental soil bacterial isolates.Microbiology (Reading, England) · 2026Article
- Recent advances in biomass deconstruction, microbial conversion, artificial intelligence, and carbon capture for sustainable bioenergy.Bioresources and bioprocessing · 2026Review
- A synergistic strategy of metabolic engineering and flocculation recycling for enhanced acetoin production inSynthetic and systems biotechnology · 2026Article
- Strategic selection of microbial cell factories for sustainable and application-specific terpenoid production.Frontiers in bioengineering and biotechnology · 2026Review
- Methanol biotransformation for the production of biodegradable plastic monomer L-lactate in yeast.Nature communications · 2025Article
- A comprehensive review of genomic-scale genetic engineering as a strategy to improve bacterial productivity.Microbiology (Reading, England) · 2025Review
- AI-powered high-throughput digital colony picker platform for sorting microbial strains by multi-modal phenotypes.Nature communications · 2025Article
- A combined pseudouridine biomanufacturing platform enabled by a streamlined designer pathway.Nature communications · 2025Article
- Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain ofSynthetic and systems biotechnology · 2025Article
- Economic and sustainable revolution to facilitate one-carbon biomanufacturing.Nature communications · 2025Review
Corrections and comments
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development and implementation of microbial chassis cells have profound impacts on circular economy. Non-model bacterium Zymomonas mobilis is an excellent chassis owing to its extraordinary industrial characteristics. Here, the genome-scale metabolic model iZM516 is improved and updated by integrating enzyme constraints to simulate the dynamics of flux distribution and guide pathway design. We show that the innate dominant ethanol pathway of Z. mobilis restricts the titer and rate of these biochemicals. A dominant-metabolism compromised intermediate-chassis (DMCI) strategy is then developed through introducing low toxicity but cofactor imbalanced 2,3-butanediol pathway, and a recombinant D-lactate producer is constructed to produce more than 140.92 g/L and 104.6 g/L D-lactate (yield > 0.97 g/g) from glucose and corncob residue hydrolysate, respectively. Additionally, techno-economic analysis (TEA) and life cycle assessment (LCA) demonstrate the commercialization feasibility and greenhouse gas reduction capability of lignocellulosic D-lactate. This work thus establishes a paradigm for engineering recalcitrant microorganisms as biorefinery chassis.
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