ArticleBiotechnology and bioengineering2025
Exploration of the Out-of-Phase Phenomenon in Shake Flasks by CFD Calculations of Volumetric Power Input, k
Article in Biotechnology and bioengineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Computational Optimization of the Orbital Shaker Operational Parameters in Endothelial Mechanobiology.Annals of biomedical engineering · 2026Article
- Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures.Journal of biological engineering · 2025Article
- Effect of High-Speed Shaking on Oxygen Transfer in Shake Flasks.Biotechnology journal · 2025Article
- Modeling and validating of oxygen transport in wave bioreactors: optimized experimental mass transfer method and novel Lattice-Boltzmann CFD approach.Frontiers in bioengineering and biotechnology · 2025Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culture broth with secreted macromolecules and culture broth of filamentous fungi showing disperse growth exhibit elevated viscosity, usually with shear-thinning flow behavior. High viscosity, however, poses a serious challenge in the production and research of these compounds and organisms. It commonly causes insufficient mixing and oxygen transfer in large- and small-scale bioreactors. Computational Fluid dynamics (CFD) has been proven to be a valuable tool for the computation of important bioprocess parameters. The published literature for small-scale shaken bioreactors, especially shake flasks, however, almost exclusively focuses on water-like viscosity. In this paper, a previously published CFD model for 250 mL shake flasks was used to simulate experiments at high viscosities of up to 100 mPa·s. Compared to experimental data, the CFD model accurately predicted the liquid distribution and computed the volumetric power input with a deviation of less than 7% and the k
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