Evidence map›Paper›PMID 39614674›Full record

ArticleCNS neuroscience & therapeutics2024

E3 Ubiquitin Ligase Ring Finger Protein 2 Alleviates Cerebral Ischemia-Reperfusion Injury by Stabilizing Mesencephalic Astrocyte-Derived Neurotrophic Factor Through Monoubiquitination.

Yujun Shen, Jinfeng Wang, Junxing Liang, Ying Chen, Xueyan Wu, Zhenhua Ren, Jiangning Zhou, Lijie Feng, Yuxian Shen

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Article in CNS neuroscience & therapeutics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Yujun ShenSchool of Basic Medical Sciences, Anhui Medical University, Hefei, China.
Jinfeng WangSchool of Basic Medical Sciences, Anhui Medical University, Hefei, China.
Junxing LiangSchool of Basic Medical Sciences, Anhui Medical University, Hefei, China.
Ying ChenSchool of Basic Medical Sciences, Anhui Medical University, Hefei, China.
Xueyan WuSchool of Basic Medical Sciences, Anhui Medical University, Hefei, China.
Zhenhua RenSchool of Basic Medical Sciences, Anhui Medical University, Hefei, China.
Jiangning ZhouAnhui Provincial Key Laboratory for Brain Bank Construction and Resource Utilization, Anhui Medical University, Hefei, China.
Lijie FengSchool of Basic Medical Sciences, Anhui Medical University, Hefei, China.
Yuxian ShenSchool of Basic Medical Sciences, Anhui Medical University, Hefei, China.ORCID 0000-0002-0916-0743

Funding

Joint Fund of the National Natural Science Foundation of China U21A20345National Natural Science Foundation of China 81301060National Natural Science Foundation of China 81973337Research Fund of Anhui Institute of translational medicine 2021zhyx-B03Research Fund of Anhui Institute of translational medicine 2023 zhyx-C01
6 · The paper itself

Abstract

aimCerebral ischemic stroke (IS) is one of the leading causes of morbidity and mortality globally. However, the mechanisms underlying IS injury remain poorly understood. Ring finger protein 2 (RNF2), the member of the polycomb family (PcG), has been implicated in diverse biological and pathological conditions. However, whether RNF2 plays a role in IS progression is not clarified. This study aims to investigate the potential effects of RNF2 on IS.

methodsThe effects of RNF2 were studied in human postmortem IS brains, a rat model of IS, tunicamycin (TM)-induced mouse neuroblastoma neuro2a (N2a) cells, and oxygen-glucose deprivation/reperfusion (OGD/R)-induced SH-SY5Y cells.

resultsHere, we demonstrated that RNF2 was markedly upregulated both in human postmortem IS brains and ischemic rat brains and RNF2 overexpression alleviated brain injury induced by middle cerebral artery occlusion by reducing neuron apoptosis. Mechanistically, we found that RNF2 is an E3 ubiquitin ligase for the mesencephalic astrocyte-derived neurotrophic factor (MANF), which confers protection against brain ischemia. RNF2 interacted with MANF and promoted the monoubiquitination of MANF, consequently facilitating its stability and nuclear localization.

conclusionCollectively, RNF2 is identified as a critical inhibitor of IS injury by stabilizing MANF through monoubiquitination, suggesting that RNF2 is a potential therapeutic target for IS.

Indexed as

Rats, Sprague-DawleyReperfusion InjuryUbiquitinationUbiquitin-Protein LigasesAnimalsApoptosisBrain IschemiaCell Line, TumorFemaleHumansInfarction, Middle Cerebral ArteryIschemic StrokeMaleMiceNerve Growth FactorsPolycomb Repressive Complex 1MANF protein, humanMANF protein, ratNerve Growth FactorsPolycomb Repressive Complex 1RNF2 protein, humanUbiquitin-Protein Ligasesischemia–reperfusion injuryischemia strokemesencephalic astrocyte‐derived neurotrophic factormonoubiquitinationring finger protein 2

Identifiers

PMID39614674
PMCPMC11607471

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