Evidence map›Paper›PMID 39614673›Full record

ArticlePhysiological reports2024

N-butylphthalide (NBP) ameliorated ischemia/reperfusion-induced skeletal muscle injury in male mice via activating Sirt1/Nrf2 signaling pathway.

Peng Lu, Wei-Peng Li, Ben-Jun Zhou, Wen-Ze Tian, Xiang Lu, Wei Gao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Physiological reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Peng LuDepartment of Geriatrics, Sir Run Run Hospital, Nanjing Medical University, Nanjing, China.
Wei-Peng LiDepartment of Geriatrics, Sir Run Run Hospital, Nanjing Medical University, Nanjing, China.
Ben-Jun ZhouDepartment of Cardiology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Wen-Ze TianDepartment of Thoracic Surgery, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, Huai'an, China.
Xiang LuDepartment of Geriatrics, Sir Run Run Hospital, Nanjing Medical University, Nanjing, China.
Wei GaoDepartment of Geriatrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.ORCID 0000-0002-4770-5062

Funding

Commissioned Project of the 2023 Special Fund for Elderly Appropriate Technology by the Jiangsu Geriatrics Society JGS2023WTSY05Jiangsu Commission of Health LKZ2023005Jiangsu Commission of Health LR2022004MOST | National Key Research and Development Program of China (NKPs) 2020YFC2008505National Natural Science Foundation of China (NSFC) 81970217
6 · The paper itself

Abstract

N-butylphthalide (NBP) has been reported to have potential protective effects in ischemic stroke via its antioxidative properties. The present study was aimed to investigate the protective effects of NBP on ischemia/reperfusion (I/R)-induced skeletal muscle injury. Mouse model of I/R-induced skeletal muscle injury and hypoxia/reoxygenation (H/R)-induced C2C12 myotube injury model were constructed to test the protective effects of NBP both in vivo and in vitro. Our results showed that I/R resulted in skeletal muscle injury, as evidenced by elevated levels of LDH, CK, ROS, 3-NT, MDA, and 4-HNE as well as decreased activities of SOD, GSH-Px, and decreased expression of Myog and MyoD in gastrocnemius muscle, which was ameliorated by NBP treatment. Mechanistically, NBP treatment increased the expression of Sirt1 and Nrf2 in the injured skeletal muscle. Notably, the protective effects of NBP on I/R-induced skeletal muscle injury was diminished by the treatment of Sirt1 inhibitor. Further studies in H/R-induced C2C12 myotubes injury model also showed that NBP activated the Sirt1/Nrf2 pathway. NBP treatment upregulated the expression of myog and MyoD in H/R-stimulated C2C12 myotubes, which was eliminated by silencing of Sirt1. Taken together, our results suggest that NBP may alleviated I/R-induced skeletal muscle injury by activating Sirt1/Nrf2 signaling pathway.

Indexed as

BenzofuransMuscle, SkeletalNF-E2-Related Factor 2Reperfusion InjurySignal TransductionSirtuin 1AnimalsCell LineMaleMiceMice, Inbred C57BLMuscle Fibers, SkeletalMyoD ProteinMyogeninOxidative Stress3-n-butylphthalideBenzofuransMyoD ProteinMyogeninMyog protein, mouseNfe2l2 protein, mouseNF-E2-Related Factor 2Sirt1 protein, mouseSirtuin 1ischemia/reperfusionN‐butylphthalideNrf2Sirt1skeletal muscle

Identifiers

PMID39614673
PMCPMC11607470

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.