Evidence map›Paper›PMID 39614143›Full record

ArticleBMC microbiology2024

Comparative genomics and virulence potential of Campylobacter coli strains isolated from different sources over 25 years in Brazil.

Carolina Nogueira Gomes, Andrei Giacchetto Felice, Giovana do Nascimento Pereira, Victor Augusto Sallum Ceballos, Siomar de Castro Soares, Ludmilla Tonani, Patrícia Helena Grizante Barião, Márcia Regina von Zeska Kress, Sheila da Silva Duque, Maria Balkey and 2 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Broad-spectrum phage cocktail targetingFrontiers in microbiology · 2026
    Article
  2. Article
  3. Small worms big discoveries:Frontiers in cellular and infection microbiology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Carolina Nogueira Gomes *Departamento de Análises Clínicas, Toxicológicas E Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Universidade de São Paulo, Ribeirão Preto/São Paulo, Brazil.
Andrei Giacchetto Felice *Departamento de Microbiologia, Imunologia E Parasitologia, Instituto de Ciências Biológicas E Naturais, Universidade Federal Do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
Giovana do Nascimento PereiraDepartamento de Análises Clínicas, Toxicológicas E Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Universidade de São Paulo, Ribeirão Preto/São Paulo, Brazil.
Victor Augusto Sallum CeballosDepartamento de Microbiologia, Imunologia E Parasitologia, Instituto de Ciências Biológicas E Naturais, Universidade Federal Do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
Siomar de Castro SoaresDepartamento de Microbiologia, Imunologia E Parasitologia, Instituto de Ciências Biológicas E Naturais, Universidade Federal Do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
Ludmilla TonaniDepartamento de Análises Clínicas, Toxicológicas E Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Universidade de São Paulo, Ribeirão Preto/São Paulo, Brazil.
Patrícia Helena Grizante BariãoDepartamento de Análises Clínicas, Toxicológicas E Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Universidade de São Paulo, Ribeirão Preto/São Paulo, Brazil.
Márcia Regina von Zeska KressDepartamento de Análises Clínicas, Toxicológicas E Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Universidade de São Paulo, Ribeirão Preto/São Paulo, Brazil.
Sheila da Silva DuqueFundação Oswaldo Cruz- FIOCRUZ, Instituto Oswaldo Cruz-IOC, Rio de Janeiro, Brazil.
Maria BalkeyDivision of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, USA.
Marc William Allard *Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, USA.
Juliana Pfrimer Falcão *Departamento de Análises Clínicas, Toxicológicas E Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto- Universidade de São Paulo, Ribeirão Preto/São Paulo, Brazil. jufalcao@fcfrp.usp.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCampylobacter spp. have been reported as a common cause of gastroenteritis in humans in many countries. However, in Brazil there is insufficient data to estimate the impact of Campylobacter in public health. In light of the importance of this foodborne pathogen, the aim of this study was to perform comparative analyses on 80 Brazilian Campylobacter coli genomes isolated from human feces, animals, the environment, and food. Methods include Average Nucleotide Identity (ANI), Gegenees, genomic plasticity, presence of pathogenicity, resistance, and metabolic islands. In addition, virulence analysis in Galleria mellonella were also performed for 18 selected C. coli strains.

resultsThe ANI values confirmed that all strains belonged to the C. coli species. Phylogenetic analyses demonstrated the evolutionary relationships among the studied strains, highlighting the genetic diversity among them. The differences in shared and deleted regions of the studied genomes were demonstrated, with 16 genomic islands identified, including 4 metabolic islands, 4 resistance islands, and 8 pathogenicity islands. We detected genes associated with chemotaxis, exotoxin production, antimicrobial resistance, stress response, defense mechanisms, and intracellular survival among these islands, highlighting the pathogenic potential of these strains. Two strains isolated from human and one from animal showed high virulence, killing 100% of Galleria mellonella larvae. Two strains isolated from the environment and two isolated from food killed 70-90% of the larvae and were classified as virulent. Three strains isolated from animal, two from human, two from the environment and one from food killed 30% to 60% of the larvae and were considered of intermediate virulence. Campylobacter jejuni ATCC 33291, one strain isolated from human and one from food killed 10 to 20% of the larvae and were considered of low virulence. One strain isolated from food did not kill any larvae and was considered avirulent.

conclusionsThe results obtained highlighted the genetic diversity, pathogenic and virulence potential of many of the C. coli strains studied, contributing for a more complete characterization of this important pathogen recognized as a cause of human gastroenteritis.

Indexed as

Campylobacter coliCampylobacter InfectionsFecesGenome, BacterialGenomic IslandsGenomicsPhylogenyAnimalsBrazilFood MicrobiologyGenetic VariationHumansLarvaMothsVirulenceVirulence FactorsVirulence FactorsCampylobacter coliGenetic diversityGenomic analysesPathogenic potentialVirulence

Identifiers

PMID39614143
PMCPMC11607955

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.