Evidence map›Paper›PMID 39614099›Full record

ArticleNature communications2024

Towards a molecular picture of the archaeal cell surface.

Matthew C Gaines, Michail N Isupov, Mathew McLaren, Clara L Mollat, Risat Ul Haque, Jake K Stephenson, Shamphavi Sivabalasarma, Cyril Hanus, Daniel Kattnig, Vicki A M Gold and 2 more

Abstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Journal of bacteriology · 2026
    Review
  2. Review
  3. Article
  4. Article
  5. How Does the Archaellum Work?Biomolecules · 2025
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Matthew C Gaines *Living Systems Institute, University of Exeter, Exeter, UK.
Michail N Isupov *Henry Wellcome Building for Biocatalysis, Department of Biosciences, Faculty of Health and Life Sciences, University of Exeter, Exeter, UK.ORCID 0000-0001-6842-4289
Mathew McLaren *Living Systems Institute, University of Exeter, Exeter, UK.ORCID 0000-0002-6636-7409
Clara L Mollat *Institute of Biology, Molecular Biology of Archaea, University of Freiburg, Freiburg, Germany.
Risat Ul HaqueLiving Systems Institute, University of Exeter, Exeter, UK.
Jake K StephensonLiving Systems Institute, University of Exeter, Exeter, UK.ORCID 0000-0001-5384-0052
Shamphavi SivabalasarmaInstitute of Biology, Molecular Biology of Archaea, University of Freiburg, Freiburg, Germany.
Cyril HanusInstitute of Psychiatry and Neurosciences of Paris, Inserm UMR1266 -Université Paris Cité, Paris, France.ORCID 0000-0002-4012-6092
Daniel KattnigLiving Systems Institute, University of Exeter, Exeter, UK.ORCID 0000-0003-4236-2627
Vicki A M GoldLiving Systems Institute, University of Exeter, Exeter, UK.ORCID 0000-0002-6908-0745
Sonja AlbersInstitute of Biology, Molecular Biology of Archaea, University of Freiburg, Freiburg, Germany.ORCID 0000-0003-2459-2226
Bertram DaumLiving Systems Institute, University of Exeter, Exeter, UK. b.daum2@exeter.ac.uk.ORCID 0000-0002-3767-264X

Funding

EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) 803894Wellcome Trust
6 · The paper itself

Abstract

Archaea produce various protein filaments with specialised functions. While some archaea produce only one type of filament, the archaeal model species Sulfolobus acidocaldarius generates four. These include rotary swimming propellers analogous to bacterial flagella (archaella), pili for twitching motility (Aap), adhesive fibres (threads), and filaments facilitating homologous recombination upon UV stress (UV pili). Here, we use cryo-electron microscopy to describe the structure of the S. acidocaldarius archaellum at 2.0 Å resolution, and update the structures of the thread and the Aap pilus at 2.7 Å and 2.6 Å resolution, respectively. We define features unique to archaella of the order Sulfolobales and compare their structure to those of Aap and threads in the context of the S-layer. We define distinct N-glycan patterns in the three filaments and identify a putative O-glycosylation site in the thread. Finally, we ascertain whether N-glycan truncation leads to structural changes in archaella and Aap.

Indexed as

Archaeal ProteinsCryoelectron MicroscopySulfolobus acidocaldariusCell MembraneFlagellaGlycosylationModels, MolecularPolysaccharidesArchaeal ProteinsPolysaccharides

Identifiers

PMID39614099
PMCPMC11607397

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.