Evidence map›Paper›PMID 39614041›Full record

ArticleMolecular biology reports2024

FoxO1 regulates human haematopoietic stem cells self-renewal and engraftment.

Lieguang Chen, Qunfang Ge, An Wu, Shasha You, Lixia Sheng, Yanli Lai, Yurong Bao, Congfa Jiang

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Lieguang ChenDepartment of Hematology, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, China.
Qunfang GeDepartment of Hematology, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, China.
An WuDepartment of Hematology, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, China.
Shasha YouDepartment of Hematology, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, China.
Lixia ShengDepartment of Hematology, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, China.
Yanli LaiDepartment of Hematology, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, China.
Yurong BaoDepartment of Hematology, The First Affiliated Hospital of Ningbo University, Ningbo, 315000, China.
Congfa JiangDepartment of Hematology, Xiangshan First People's Hospital Medical Health Group, Ningbo, 315700, China. mz1581xyk@163.com.

Funding

Zhejiang medicine and health science and technology project 2024KY1529
6 · The paper itself

Abstract

backgroundHematopoietic stem cell transplantation (HSCT) is one of the most effective ways to treat hematological malignant diseases, but the traditional culture of hematopoietic stem cells (HSCs) in vitro will soon lose their ability to self-renewal or differentiate into multilineage blood cells.

methodsTo determine whether Forkhead boxO1 (FoxO1) is implicated in the development of HSCs, lentiviral vectors expressing knockdown (KD) or overexpression (OE) of FoxO1 were utilized in fetal liver-derived hematopoietic stem and progenitor cells (FL-HSPCs). The impacts on the proliferation and hematopoietic differentiation of FL-HSPCs were subsequently evaluated via flow cytometry (FCM). Furthermore, the effect of FoxO1-OE on the self-renewal of cord blood-derived hematopoietic stem and progenitor cells (CB-HSPCs) was investigated. Additionally, the transplantation ability of hematopoietic stem cells derived from these CB-HSPCs in mice after secondary transplantation was also assessed by FCM.

resultAfter knocking down FoxO1 in FL-HSPCs, the apoptosis rate was significantly increased, and the expression of hCD45 was significantly decreased. Conversely, overexpression of FoxO1 reversed this phenomenon, effectively promoting the expansion and differentiation of FL-HSPCs in vitro. Similarly, it was found that FoxO1-OE could effectively enhance the expansion of CB-HSPCs. Furthermore, upon transplantation of CB-HSPCs overexpressing FoxO1 into NSG mice, multilineage human hematopoietic reconstruction was promoted. Notably, the results of secondary transplantation revealed that only the FoxO1-OE group exhibited multilineage reconstitution.

conclusionIn conclusion, our study confirmed that FoxO1-OE could enhance the self-renewal and engraftment of CB-HSPCs.

Indexed as

Cell DifferentiationCell ProliferationCell Self RenewalForkhead Box Protein O1Hematopoietic Stem CellsHematopoietic Stem Cell TransplantationAnimalsApoptosisFetal BloodHumansMiceForkhead Box Protein O1FOXO1 protein, humanDifferentiationEngraftmentFoxO1Hematological malignant diseasesSelf-renewal

Identifiers

PMID39614041

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.