Evidence map›Paper›PMID 39613390›Full record

ArticleBMJ open ophthalmology2024

Embryoid body-based differentiation of human-induced pluripotent stem cells into cells with a corneal stromal keratocyte phenotype.

Jie Chen, Qingjian Ou, Yifan Liu, Tingting Cui, Huimin Yang, Jiancen Tang, Lixia Lu, Guotong Xu, Hongping Cui, Caixia Jin and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMJ open ophthalmology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Stem cell therapy as treatment for Stargardt disease.Therapeutic advances in ophthalmology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jie ChenDepartment of Ophthalmology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.ORCID 0009-0001-7108-9650
Qingjian OuDepartment of Ophthalmology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Yifan LiuDepartment of Ophthalmology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Tingting CuiDepartment of Ophthalmology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Huimin YangDepartment of Ophthalmology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Jiancen TangDepartment of Ophthalmology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
Lixia LuDepartment of Ophthalmology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Guotong XuDepartment of Ophthalmology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Hongping CuiDepartment of Ophthalmology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
Caixia JinDepartment of Ophthalmology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Qian LiDepartment of Ophthalmology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China liliqq@tongji.edu.cn.ORCID 0009-0001-9157-2375

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe transparency of the cornea is determined by the extracellular matrix, which is secreted by corneal stromal keratocytes (CSKs). Human-induced pluripotent stem cell (hiPSC)-derived keratocytes (hiPSC-CSKs) can be used in cell-based therapy for treating corneal blindness. Our goal was to develop an effective small molecule-based technique for differentiating hiPSCs into keratocytes. METHODS AND ANALYSIS: hiPSCs were cultured in chemically defined medium, and embryoid bodies (EBs) were generated; these EBs were induced into CSKs using keratocyte-differentiated medium. The expression of keratocyte-specific markers was assessed using quantitative RT-PCR, immunostaining and Western blotting.

resultsWe found that the expression of genes encoding keratocyte markers, including aldehyde dehydrogenase 1 family member A1 (ALDH1A1), lumican and keratocan, was upregulated. Immunostaining showed positive staining for ALDH1A1 and keratocan in the hiPSC-CSK samples. Similarly, western blot analysis indicated that ALDH1A1 and keratocan expression levels were significantly greater in the hiPSC-CSKs than in the control cells. In addition, hiPSC-CSKs were not transformed into fibroblasts or myofibroblasts.

conclusionWe established an innovative and effective method to generate CSKs via the EB-based differentiation of hiPSCs, which might be employed for cell-based therapy of corneal stromal opacities.

Indexed as

Cell DifferentiationCorneal KeratocytesCorneal StromaEmbryoid BodiesInduced Pluripotent Stem CellsPhenotypeAldehyde Dehydrogenase 1 FamilyBiomarkersBlotting, WesternCells, CulturedHumansLumicanProteoglycansReal-Time Polymerase Chain ReactionRetinal DehydrogenaseAldehyde Dehydrogenase 1 FamilyALDH1A1 protein, humanBiomarkersKERA protein, humanLumicanLUM protein, humanProteoglycansRetinal DehydrogenaseCorneaStem Cells

Identifiers

PMID39613390
PMCPMC11605830

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.