Evidence map›Paper›PMID 39611913›Full record

ArticleAsian Pacific journal of cancer prevention : APJCP2024

Anticancer Properties of Garlic and Ginger Extract in Colon Cancer Cell Line.

Aysam M Fayed, Habeeb S N, Walaa Samy, Khalid Bassiouny, Amal A Abd-El-Aziz, Dina M R AlKhafaf, Hasanain Khaleel Shareef, Mohamed AbdElrahman, Ahmed Khalid Aldhalmi, Dalya S Obaida and 2 more

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In one paragraph

Article in Asian Pacific journal of cancer prevention : APJCP, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Aysam M FayedMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.
Habeeb S NMedical Laboratory Techniques Department, College of Health and Medical Technique, Al-Mustaqbal University, Babylon, Iraq.
Walaa SamyMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.
Khalid BassiounyMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.
Amal A Abd-El-AzizMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.
Dina M R AlKhafafMedical Laboratory Techniques Department, College of Health and Medical Technique, Al-Mustaqbal University, Babylon, Iraq.
Hasanain Khaleel ShareefUniversity of Babylon, College of Science for Women, Biology Department, Iraq.
Mohamed AbdElrahmanCollege of Pharmacy, Al-Mustaqbal University, Babylon,51001, Iraq.
Ahmed Khalid AldhalmiCollege of Pharmacy, Al-Mustaqbal University, Babylon,51001, Iraq.
Dalya S ObaidaMedical Laboratory Techniques Department, College of Health and Medical Technique, Al-Mustaqbal University, Babylon, Iraq.
H KhalilMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.
Ahmed Abd ElbadeeAnimal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colon cancer typically affects older adults, though it can happen at any age. Colon cancer, also known as Caco-2, is caused by multiple epigenetic alterations and involves unregulated proliferation, differentiation, and invasion of neighboring tissues. Colon cancer patients have had surgery, radiation, hormone therapy, and chemotherapy. This study investigates a new experimental method using inexpensive and environmentally friendly Egyptian plant extracts. DMSO-dissolved ginger, garlic, cinnamon, and chamomile were employed in this investigation. HPLC and GC-MS were used to analyze plant extracts. These extracts were tested for colon cancer efficacy using various methods. These methods included Caco-2 cells, MTT test, Annexin V-FITC flow cytometry, qRT-PCR, and ELISA. Garlic and ginger were found to be cytotoxic to Caco-2 cells. Compared to cinnamon and chamomile extracts, garlic and ginger have boosted LDH synthesis significantly. Garlic and ginger also altered autophagy genes (Bectin1, Atg5, PTEN) and Caspase-3 expression pathways on proapoptotic signaling. Garlic and ginger increased cleaved PTEN and caspase-3 and decreased Atg5 and Bectin1. Ginger and garlic caused extrinsic apoptosis and prevented Atg5 and Bectin1 phosphorylation. The average IL-8 and IL-6 levels increased significantly after 24 hours, according to ELISA. In conclusion, garlic and ginger extracts modify pro-inflammatory cytokines. Alternative herbal remedies like garlic and ginger may be effective and safe colon cancer treatments.

Indexed as

ApoptosisCell ProliferationColonic NeoplasmsGarlicPlant ExtractsZingiber officinaleAutophagyCaco-2 CellsCinnamomum zeylanicumHumansTumor Cells, CulturedPlant ExtractsAtg5Bectin1IL-6IL-8PTEN

Identifiers

PMID39611913
PMCPMC11996100

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.