Evidence map›Paper›PMID 39611745›Full record

ArticleFuture microbiology2025

Detection of MPT-64 protein in pleural tuberculosis cases by magnetic bead-gold nanoparticle-PCR amplified immunoassay.

Aishwarya Soni, Kiran Nehra, Bhawna Dahiya, Anam Rais, Tulika Prasad, Anjum Gahlaut, Vikas Raj, Reetu Sheoran, Aparna Parmar, Promod K Mehta

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Article in Future microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Aishwarya SoniMicrobiology Department, Faculty of Allied Health Sciences (FAHS), Shree Guru Gobind Singh Tricentenary (SGT) University, Gurugram, India.
Kiran NehraDepartment of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology (DCRUST), Sonipat, India.
Bhawna DahiyaMicrobiology Department, Faculty of Allied Health Sciences (FAHS), Shree Guru Gobind Singh Tricentenary (SGT) University, Gurugram, India.
Anam RaisSpecial Centre for Nano Science and Advanced Instrumentation Research and Facility, Jawaharlal Nehru University, New Delhi, India.
Tulika PrasadSpecial Centre for Nano Science and Advanced Instrumentation Research and Facility, Jawaharlal Nehru University, New Delhi, India.
Anjum GahlautCentre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, India.
Vikas RajCentre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, India.
Reetu SheoranSchool of Basic Sciences and Research, Sharda University, Greater Noida, India.
Aparna ParmarDepartment of Microbiology, University of Health Sciences (UHS), Rohtak, India.
Promod K MehtaMicrobiology Department, Faculty of Allied Health Sciences (FAHS), Shree Guru Gobind Singh Tricentenary (SGT) University, Gurugram, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimDiagnosis of pleural tuberculosis (TB) is challenging; thus, an efficient method is urgently needed.

methodsWe developed a magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR, liquid system) to detect the

resultsWe utilized the MB-AuNP-I-PCR for MPT-64 detection in 99 clinical specimens which displayed 85.2% sensitivity and 97.8% specificity to diagnose pleural TB cases. Markedly, the sensitivity achieved by MB-AuNP-I-PCR was noticeably higher (

conclusionThis is a preliminary report to diagnose pleural TB cases by MB-AuNP-I-PCR with promising results that require further corroboration in a higher number of specimens.

Indexed as

Antigens, BacterialBacterial ProteinsMetal NanoparticlesMycobacterium tuberculosisPolymerase Chain ReactionTuberculosis, PleuralEnzyme-Linked Immunosorbent AssayGoldHumansImmunoassaySensitivity and SpecificityAntigens, BacterialBacterial ProteinsGoldMPT64 protein, Mycobacterium tuberculosisDiagnosisfunctionalized AuNPsGeneXpertmagnetic beadsMagneto-ELISAMB-AuNP-I-PCRMPT-64pleural TB

Identifiers

PMID39611745
PMCPMC11792811

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