ArticleNucleic acids research2024
Molecular basis of the phosphorothioation-sensing antiphage defense system IscS-DndBCDE-DndI.
Article in Nucleic acids research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Phage hijacks host phosphorothioate DNA modification machinery to circumvent bacterial Ssp defences.Nature microbiology · 2026Article
- Article
- Dendrobium officinale leaf extract extends the mean lifespan in Caenorhabditis elegans via the DAF-16/SOD-3 axis.Biogerontology · 2026Article
- Characterization of defensome genes and mobile genetic Elements in different types of pasture soil agroecosystems from the Brazilian Amazon.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Prophage: agent provocateur?Archives of microbiology · 2026Review
- Fucoidan induces ferroptosis in colorectal cancer cells by regulating the SLC7A11/GPX4 signaling pathway and modulating metabolic pathways.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Facile solid-liquid biphasic PCR platform for ultrasensitive multiplex detection and genotyping of HPV.Mikrochimica acta · 2025Article
- Article
- PglZ from Type I BREX phage defence systems is a metal-dependent nuclease that forms a sub-complex with BrxB.Nucleic acids research · 2025Article
- Temporal dynamics and metagenomics of phosphorothioate epigenomes in the human gut microbiome.Microbiome · 2025Article
- Microbial production systems and optimization strategies of antimicrobial peptides: a review.World journal of microbiology & biotechnology · 2025Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Phosphorothioation serves as a DNA backbone modification mechanism, wherein a sulfur atom substitutes the nonbridging oxygen atom within the phosphodiester, facilitated by the gene products of dndABCDE or sspABCD. The combination of dndABCDE with dndFGH forms a bona fide defense system, where the DndFGH protein complex exhibits DNA nickase and DNA translocase activities to prevent phage invasion. In this study, we identified that dndI, co-transcribed with dndFGH, can independently couple with iscS-dndBCDE as an anti-phage defense system. Moreover, we resolved the crystal structure of DndI from Salmonella at a resolution of 3.10 Å. We discovered that its residue Y25, residing within a hydrophobic region of DndI, is involved in phosphorothioate (PT) sensing. Upon sensing PT modifications at 5'-GPSAAC-3'/5'-GPSTTC-3', the ATPase activity of DndI is stimulated, which subsequently triggers a conformational transition, facilitating the dissociation of DndI from self-DNA, thereby allowing DndI to avoid cleaving self-DNA while restricting PT-deficient phage DNA. This research broadens the knowledge of the mechanistic diversity underlying PT-based defense systems and highlights their complexity in the course of evolution.
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