Evidence map›Paper›PMID 39609820›Full record

ArticleBMC veterinary research2024

Antimicrobial resistance, virulence genes profiles and molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae strains from captive giant pandas (Ailuropoda melanoleuca).

Xia Yan, Mei Yang, James Edward Ayala, Lin Li, Yang Zhou, Rong Hou, Songrui Liu, Yunli Li, Chanjuan Yue, Dongsheng Zhang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xia YanChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
Mei YangChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
James Edward AyalaChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
Lin LiChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
Yang ZhouChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
Rong HouChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
Songrui LiuChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
Yunli LiChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
Chanjuan YueChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
Dongsheng ZhangChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China.
Xiaoyan SuChengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, 610081, China. xyansu@126.com.

Funding

Chengdu Research Base of Giant Panda Breeding 2021CPB-B15Chengdu Research Base of Giant Panda Breeding 2024CPB-B11
6 · The paper itself

Abstract

backgroundCarbapenem-resistant Klebsiella pneumoniae (CRKP) increases the difficulty of clinical treatment of giant pandas. This study aimed to investigate the antibiotic susceptibility, antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), virulence genes, and molecular epidemiology of CRKP strains isolated from giant pandas. A total of 187 nonduplicated Klebsiella pneumoniae (KP) isolates were collected from fresh feces of captive giant pandas at the Chengdu Research Base of Giant Panda Breeding. Then CRKP were isolated and identified through carbapenase Carba NP assay. Subsequently, the antimicrobial susceptibility testing and antibiotic resistance genes of CRKP isolates were studied by disk diffusion (K-B) and HT-qPCR, respectively. Then both the MGEs and virulence genes of CRKP isolates were analyzed by PCR. In addition, molecular epidemiology was analyzed among the CRKP strains using pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST).

resultsEight strains of CRKP (4.5%) were isolated and identified among the 187 KP strains, and seven of eight CRKP strains both exhibited resistance to imipenem, while one strain showed resistance to meropenem, and one demonstrated multiple resistance; eight CRKP strains carried a large amount of ARGs, among which amp

conclusionsThis study highlighted the diversity of CRKP strains isolated from giant panda feces, which exhibited varying levels of antibiotic resistance along with multiple ARGs, MGEs and virulence genes present. These findings emphasized the importance of monitoring and researching antibiotic resistance within wildlife populations to protect the health status of these conservation dependent animals.

Indexed as

Anti-Bacterial AgentsKlebsiella InfectionsKlebsiella pneumoniaeMolecular EpidemiologyUrsidaeAnimalsCarbapenemsDrug Resistance, BacterialElectrophoresis, Gel, Pulsed-FieldMicrobial Sensitivity TestsMultilocus Sequence TypingVirulenceVirulence FactorsAnti-Bacterial AgentsCarbapenemsVirulence FactorsAntimicrobial resistanceCarbapenem-resistant Klebsiella pneumoniaeMobile genetic elementsMolecular epidemiologyVirulence genes

Identifiers

PMID39609820
PMCPMC11603722

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.