Evidence map›Paper›PMID 39607507›Full record

ArticleAnalytical and bioanalytical chemistry2025

Continuous chromatography system with 6-zone and 18-column dynamic tandem connection technique for the enrichment of total flavonoids from Epimedium koreanum Nakai.

Yi Cui, Jie Huai, Yiting Cai, Shuai Li, Weiye Zhang, Taoyulin Zhou, Yujie Song, Han Zhang, Shengfu Li, Linwu Zhuang and 1 more

Abstract read
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Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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5 · Who and what money

Authors and funding

11 authors.

Yi CuiJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China.
Jie HuaiJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China.
Yiting CaiJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China.
Shuai LiJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China.
Weiye ZhangJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China.
Taoyulin ZhouJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China.
Yujie SongJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China.
Han ZhangJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China.
Shengfu LiJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China.
Linwu ZhuangJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China. zhuanglinwu@jou.edu.cn.ORCID http://orcid.org/0000-0003-0831-0065
Junjie ZhangJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, PR China. zjj203204@163.com.

Funding

the Innovation Ability Improvement Project of Science and Technology smes in Shandong Province No. 2022TSGC2548the Postgraduate Research & Practice Innovation Program of Jiangsu Ocean University No. KYCX2023-134the Research Fund Project for Talent Introduction of Jiangsu Ocean University No. 2022TSGC2548
6 · The paper itself

Abstract

Natural flavonoids have been shown to have many pharmacological activities. Efficient and continuous enrichment of total flavonoids with high content and low mobile phase usage from complex natural products is greatly needed at the moment. In this study, a new continuous chromatography system (CCS) with 6 zones and the 18-column dynamic tandem connection technique was developed and used to enrich total flavonoids from Epimedium koreanum Nakai (EKN). The 18 columns were divided into 6 zones, and the principle of a dynamic series of three columns was adopted for each zone to achieve continuous automatic separation and enrichment of total flavonoids under the control of a logic control valve. The CCS separation conditions were established based on single-column chromatography and a theoretical calculation model of the CCS. By means of the self-designed device and method, 485.11±3.16 g of total flavonoids were isolated from 16.2 kg of EKN. It is worth noting that the total content of 18 types of flavonoids in the samples enriched by the CCS was increased from 2.84±0.07% to 88.29±0.22%, the total recovery rate was 92.20±0.38%, and the RSD of each flavonoid was less than 5.0%. Furthermore, compared with single-column chromatography filled with the same volume of chromatography filler, the entire process saved about 2/3 of the mobile phase usage. In summary, the developed device and method could efficiently and continuously enrich total flavonoids from EKN with high-content and low mobile phase usage and would have a wide application prospect in the separation and enrichment of natural products.

Indexed as

EpimediumFlavonoidsChromatography, High Pressure LiquidFlavonoidsContinuous chromatography systemDynamic seriesEnrichmentEpimedium koreanum NakaiTotal flavonoids

Identifiers

PMID39607507

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