Evidence map›Paper›PMID 39605783›Full record

ArticleJournal of advanced veterinary and animal research2024

Modification of cryoprotectants on sperm cryopreservation: A study of embryo development.

Manggiasih Dwiayu Larasati, Silvia Werdhy Lestari, Mulyoto Pangestu, Andon Hestiantoro, Gito Wasian

Abstract read
In one paragraph

Article in Journal of advanced veterinary and animal research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Enhancing cryopreservation performance in semen quality of Bali bulls (Journal of advanced veterinary and animal research · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Manggiasih Dwiayu LarasatiDoctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.ORCID https://orcid.org/0000-0003-2053-6745
Silvia Werdhy LestariDepartment of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.ORCID https://orcid.org/0000-0003-3433-1097
Mulyoto PangestuEducation Program in Reproduction and Development, Department of Obstetrics and Gynecology, School of Clinical Sciences, Monash University, Melbourne, VIC, Australia.ORCID https://orcid.org/0000-0001-5623-3995
Andon HestiantoroDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.ORCID https://orcid.org/0000-0001-7424-679X
Gito WasianDepartment of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.ORCID https://orcid.org/0000-0001-8538-0003

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: This research was conducted to analyze the effect of cryopreservation of sperm using modified cryoprotectants on embryo development through Materials and Methods: Several parameters, namely sperm concentration, motility, morphology, plasma membrane integrity, cryo-survival rate, and viability rate, were measured and compared before vitrification and warming. Embryo development was also observed on the first and third days of development based on the cell number, cell size, and fragmentation rate. Results: Sperm cryopreservation exhibited a negative influence on embryo quality. Both Nakagata cryoprotectants and modified cryoprotectants attained good-quality embryos. In terms of embryonic development, this research revealed a modified cryoprotectant superior to Nakagata's cryoprotectant, although Kitazato was superior to the Nakagata cryoprotectant and modified cryoprotectant. Significant differences were found in the three cryoprotectants on observations on day 1 and day 3, all of them with Conclusion: Modified cryoprotectant was found to be better than Nakagata but less significant than Kitazato in terms of embryonic development quality. Therefore, modified cryoprotectants could be a better alternative compared to commercial (Kitazato) cryoprotectants for improving embryo quality.

Indexed as

Cryoprotectant modificationembryo developmentsperm cryopreservation

Identifiers

PMID39605783
PMCPMC11590591

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.