Evidence map›Paper›PMID 39605704›Full record

ArticlebioRxiv : the preprint server for biology2024

CRISPRi-based screen of Autism Spectrum Disorder risk genes in microglia uncovers roles of

Olivia M Teter, Amanda McQuade, Venus Hagan, Weiwei Liang, Nina M Dräger, Sydney M Sattler, Brandon B Holmes, Vincent Cele Castillo, Vasileios Papakis, Kun Leng and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Olivia M TeterInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Amanda McQuadeInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Venus HaganInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Weiwei LiangInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Nina M DrägerInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Sydney M SattlerInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Brandon B HolmesDepartment of Neurology, University of California, San Francisco, CA, USA.
Vincent Cele CastilloInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Vasileios PapakisInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Kun LengInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Steven BoggessInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.
Tomasz J NowakowskiDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
James WellsDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Martin KampmannInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, CA, USA.ORCID 0000-0002-3819-7019

Funding

The Psychiatric Cell Map Initiative: Connecting Genomics, Subcellular Networks, and Higher Order PhenotypesU01MH115747 · NIMH · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KAMPMANN, MARTIN, KROGAN, NEVAN J · 2018 to 2022
$19.6M
PREDOCTORAL TRAINING IN DEVELOPMENTAL BIOLOGYT32HD007470 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Todd Nystul · 1994 to 2026
$7.7M
Assessing Genomic, Regulatory and Transcriptional Variation at Single Nuclei Resolution in the Brains of Individuals with Autism Spectrum DisorderR01MH125516 · NIMH · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ARNOLD KRIEGSTEIN, Tomasz Nowakowski · 2021 to 2026
$3.8M
Directed Evolution of Novel AAVs and Regulatory Elements for Selective Microglial Gene ExpressionR01NS126397 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Tomasz Nowakowski, DAVID V SCHAFFER · 2023 to 2026
$3.2M
NICHD NIH HHS T32 HD007470NIMH NIH HHS R01 MH125516NIMH NIH HHS U01 MH115747NINDS NIH HHS R01 NS126397
6 · The paper itself

Abstract

Autism Spectrum Disorders (ASD) are a set of neurodevelopmental disorders with complex biology. The identification of ASD risk genes from exome-wide association studies and de novo variation analyses has enabled mechanistic investigations into how ASD-risk genes alter development. Most functional genomics studies have focused on the role of these genes in neurons and neural progenitor cells. However, roles for ASD risk genes in other cell types are largely uncharacterized. There is evidence from postmortem tissue that microglia, the resident immune cells of the brain, appear activated in ASD. Here, we used CRISPRi-based functional genomics to systematically assess the impact of ASD risk gene knockdown on microglia activation and phagocytosis. We developed an iPSC-derived microglia-neuron coculture system and high-throughput flow cytometry readout for synaptic pruning to enable parallel CRISPRi-based screening of phagocytosis of beads, synaptosomes, and synaptic pruning. Our screen identified

Identifiers

PMID39605704
PMCPMC11601228

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.