Evidence map›Paper›PMID 39605575›Full record

ArticlebioRxiv : the preprint server for biology2024

Physical vitrification and nanowarming at human organ scale to enable cryopreservation.

Lakshya Gangwar, Zonghu Han, Cameron Scheithauer, Bat-Erdene Namsrai, Saurin Kantesaria, Rob Goldstein, Michael L Etheridge, Erik B Finger, John C Bischof

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Lakshya GangwarDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, USA.
Zonghu HanDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, USA.
Cameron ScheithauerDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, USA.
Bat-Erdene NamsraiDepartment of Surgery, University of Minnesota, Minneapolis, USA.
Saurin KantesariaDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, USA.
Rob GoldsteinAMF Life Systems, LLC, Auburn Hills, USA.
Michael L EtheridgeDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, USA.
Erik B FingerDepartment of Surgery, University of Minnesota, Minneapolis, USA.
John C BischofDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, USA.

Funding

Organ banking for transplant—kidney cryopreservation by vitrification and novel nanowarming technologyR01DK117425 · NIDDK · UNIVERSITY OF MINNESOTA · PI JOHN C BISCHOF, Erik Brian Finger · 2018 to 2026
$4.9M
Cryopreservation and nanowarming enables whole liver banking for transplantation, cell therapy and biomedical researchR01DK132211 · NIDDK · UNIVERSITY OF MINNESOTA · PI JOHN C BISCHOF, Erik Brian Finger · 2023 to 2026
$2.6M
Breakthrough Tissue and Organ Preservation and Transplantation Using Scaled-Up Nanowarming TechnologyR01HL135046 · NHLBI · UNIVERSITY OF MINNESOTA · PI BISCHOF, JOHN C, FINGER, ERIK BRIAN · 2017 to 2020
$2.6M
NHLBI NIH HHS R01 HL135046NIDDK NIH HHS R01 DK117425NIDDK NIH HHS R01 DK132211
6 · The paper itself

Abstract

Organ banking by vitrification could revolutionize transplant medicine. However, vitrification and rewarming have never been demonstrated at the human organ scale. Using modeling and experimentation, we tested the ability to vitrify and rewarm 0.5 - 3 L volumes of three common cryoprotective agent (CPA) solutions: M22, VS55, and 40% EG+0.6M Sucrose. We first demonstrated our ability to avoid ice formation by convectively cooling faster than the critical cooling rates of these CPAs while also maintaining adequate uniformity to avoid cracking. Vitrification success was then verified by visual, thermometry, and x-ray μCT inspection. M22 and EG+sucrose were successfully vitrified in 0.5 L bags, but only M22 was vitrified at 3 L. VS55 did not vitrify at any tested volumes. As additional proof of principle, we successfully vitrified a porcine liver (~1L) after perfusion loading with 40% EG+0.6M Sucrose. Uniform volumetric rewarming was then achieved in up to 2 L volumes (M22 with ~5 mgFe/mL iron-oxide nanoparticles) using nanowarming, reaching a rate of ~88 °C/min with a newly developed 120 kW radiofrequency (RF) coil operating at 35kA/m and 360kHz. This work demonstrates that human organ scale vitrification and rewarming is physically achievable, thereby contributing to technology that enables human organ banking.

Indexed as

CryopreservationHuman OrganLiter volumesNanowarmingVitrification

Identifiers

PMID39605575
PMCPMC11601234

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.