Evidence map›Paper›PMID 39605319›Full record

ArticlebioRxiv : the preprint server for biology2025

Versatile and Portable Cas12a-mediated Detection of Antibiotic Resistance Markers.

Maryhory Vargas-Reyes, Roberto Alcántara, Soraya Alfonsi, Katherin Peñaranda, Dezemona Petrelli, Roberto Spurio, Monica J Pajuelo, Pohl Milon

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Maryhory Vargas-ReyesBiomolecules Laboratory, School of Biology, Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru.
Roberto AlcántaraBiomolecules Laboratory, School of Biology, Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru.ORCID 0000-0003-2028-631X
Soraya AlfonsiSchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Katherin PeñarandaBiomolecules Laboratory, School of Biology, Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru.
Dezemona PetrelliSchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Roberto SpurioSchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Monica J PajueloLaboratorio de Microbiología Molecular, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias e Ingeniería, Universidad Peruana Cayetano Heredia, Lima, Peru.
Pohl MilonBiomolecules Laboratory, School of Biology, Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru.ORCID 0000-0001-6679-5473

Funding

Training in Parasitic Diseases and Central Nervous System Infections in Peru.D43TW001140 · FIC · UNIVERSIDAD PERUANA CAYETANO HEREDIA · PI GARCIA, HECTOR HUGO, GILMAN, ROBERT H · 1999 to 2025
$5.7M
FIC NIH HHS D43 TW001140
6 · The paper itself

Abstract

Antibiotic-resistant bacteria are spreading in clinical, industrial, and environmental ecosystems. The spreading dynamics to and from the environment are unknown, largely due to the lack of appropriate (robust, fast, low-cost) analytical assays. In this study, we developed C12a, a versatile molecular toolbox to detect genetic markers of antibiotic resistance using CRISPR/Cas12a. Biochemical characterization show that the C12a toolbox can detect less than 100 attoMolar of pure DNA fragments from the

Indexed as

Antibiotic Resistance GeneblaCTXCas12afloRIntegrase

Identifiers

PMID39605319
PMCPMC11601430

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.