Evidence map›Paper›PMID 39604403›Full record

ArticleScientific reports2024

A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense.

Pengfei Cai, Wenyi Zhang, Sufei Jiang, Yiwei Xiong, Hui Qiao, Shubo Jin, Hongtuo Fu

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Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Pengfei CaiWuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
Wenyi ZhangKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
Sufei JiangKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
Yiwei XiongKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
Hui QiaoKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
Shubo JinWuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China. jinsb@ffrc.cn.
Hongtuo FuWuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China. fuht@ffrc.cn.

Funding

Jiangsu Agricultural Industry Technology System; Central Public-Interest Scientific Institution Basal Research Fund CAFS (2023TD39, 2023JBFM04)the earmarked fund CARS-48-07the Natural Science Foundation of Jiangsu Province BK20221207the seed industry revitalization project of Jiangsu province JBGS [2021] 118
6 · The paper itself

Abstract

Sex differentiation in crustaceans is a complex process influenced by various factors, including the androgenic gland and sex-related genes. This study characterized the role of the Mn-DHCR24 gene in the oriental river prawn (Macrobrachium nipponense). We used bioinformatics to analyze sequence features and phylogenetic relationships of a single Mn-DHCR24 gene. The expression patterns of Mn-DHCR24 across different tissues and developmental stages were determined by real-time PCR, and its localization in testis was determined by in situ hybridization. RNA interference was used to knock down Mn-DHCR24 expression, followed by examining changes in sex ratio and gonadal development at the PL10 stage. Additionally, an enzyme-linked immunosorbent assay measured 17α-methyltestosterone levels, and tissue sections were used to characterize gonadal development. The results indicated that Mn-DHCR24 was high expression in testis, which was critical for sperm maturation and gonadal differentiation. RNAi experiments showed the role of Mn-DHCR24 during reproductive regulation rather than as a master gene for sex differentiation. This study further showed that Mn-DHCR24 regulated sex and hormone-related genes, influencing steroid biosynthesis pathways. Together, these findings provided valuable insights into the genetic and hormonal mechanisms of gonadal differentiation in M. nipponense, and supported the development of monosex culture technology.

Indexed as

PalaemonidaeSex DifferentiationTestisAnimalsArthropod ProteinsFemaleGene Expression Regulation, DevelopmentalGonadsMalePhylogenyRNA InterferenceArthropod ProteinsGonadal developmentMacrobrachium nipponenseMn-DHCR24RNAiSex differentiationSex hormone

Identifiers

PMID39604403
PMCPMC11603370

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.