Evidence map›Paper›PMID 39604360›Full record

ArticleNature communications2024

Single-cell transcriptome analysis reveals CD34 as a marker of human sinoatrial node pacemaker cardiomyocytes.

Amos A Lim, Delaram Pouyabahar, Mishal Ashraf, Kate Huang, Michelle Lohbihler, Brandon M Murareanu, Matthew L Chang, Maggie Kwan, Faisal J Alibhai, Thinh Tran and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Amos A LimMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Delaram PouyabaharDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Mishal AshrafCentre for Heart and Lung Innovation, University of British Columbia and St. Paul's Hospital, Vancouver, BC, Canada.
Kate HuangCentre for Heart and Lung Innovation, University of British Columbia and St. Paul's Hospital, Vancouver, BC, Canada.ORCID 0000-0002-6515-5324
Michelle LohbihlerMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Brandon M MurareanuMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Matthew L ChangMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Maggie KwanMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Faisal J AlibhaiMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Thinh TranDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Amine MazineMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Michael A LaflammeMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Gary D BaderDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada.ORCID 0000-0003-0185-8861
Zachary LaksmanCentre for Heart and Lung Innovation, University of British Columbia and St. Paul's Hospital, Vancouver, BC, Canada.ORCID 0000-0002-7790-541X
Stephanie ProtzeMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada. stephanie.protze@uhn.ca.ORCID 0000-0002-7993-3780

Funding

Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) CIHR PJT 169090Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) Doctoral FellowshipGouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) Postdoctoral Fellowship
6 · The paper itself

Abstract

The sinoatrial node regulates the heart rate throughout life. Failure of this primary pacemaker results in life-threatening, slow heart rhythm. Despite its critical function, the cellular and molecular composition of the human sinoatrial node is not resolved. Particularly, no cell surface marker to identify and isolate sinoatrial node pacemaker cells has been reported. Here we use single-nuclei/cell RNA sequencing of fetal and human pluripotent stem cell-derived sinoatrial node cells to reveal that they consist of three subtypes of pacemaker cells: Core Pacemaker, Sinus Venosus, and Transitional Cells. Our study identifies a host of sinoatrial node pacemaker markers including MYH11, BMP4, and the cell surface antigen CD34. We demonstrate that sorting for CD34

Indexed as

Antigens, CD34Cell DifferentiationMyocytes, CardiacSingle-Cell AnalysisSinoatrial NodeBiomarkersGene Expression ProfilingHumansInduced Pluripotent Stem CellsPluripotent Stem CellsSingle-Cell Gene Expression AnalysisTranscriptomeAntigens, CD34Biomarkers

Identifiers

PMID39604360
PMCPMC11603134

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.