Evidence map›Paper›PMID 39601042›Full record

ArticleEpigenomics

Dynamic visualization of DNA methylation in cell cycle genes during iPSC cardiac differentiation.

Ning Li, Ba Thong Nguyen, Edward A Stitt, Zhenhe Zhang, W Robb MacLellan, Yiqiang Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epigenomics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Ning LiDivision of Cardiology, Department of Medicine, University of Washington, Seattle, WA, USA.
Ba Thong NguyenDepartment of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA.
Edward A StittDepartment of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA.
Zhenhe ZhangDivision of Cardiology, Department of Medicine, University of Washington, Seattle, WA, USA.
W Robb MacLellanDivision of Cardiology, Department of Medicine, University of Washington, Seattle, WA, USA.
Yiqiang ZhangDivision of Cardiology, Department of Medicine, University of Washington, Seattle, WA, USA.ORCID 0000-0003-0691-6645

Funding

The Role of gp120 on Cardiovascular Disease in People Living with HIVU54MD007601 · NIMHD · UNIVERSITY OF HAWAII AT MANOA · PI JoAnn Umilani Tsark · 2017 to 2026
$59.5M
TRPA1 Physiology in Diabetes, Metabolic Syndrome, and MetabolismP20GM113134 · NIGMS · UNIVERSITY OF HAWAII AT MANOA · PI Kathryn Jaques Schunke · 2017 to 2026
$21.5M
Productive and Leisure Activities and Cognition in Later LifeP20GM125526 · NIGMS · KUAKINI MEDICAL CENTER · PI LEE, YEONJUNG · 2019 to 2023
$12.0M
Epigenetic Mechanisms of Myocardial RegenerationR56HL131898 · NHLBI · UNIVERSITY OF WASHINGTON · PI ZHANG, YIQIANG · 2016 to 2016
$438k
NHLBI NIH HHS R56 HL131898NIGMS NIH HHS P20 GM113134NIGMS NIH HHS P20 GM125526NIMHD NIH HHS U54 MD007601
6 · The paper itself

Abstract

backgroundExisting analyses with conventional assays have generated significant insights into static states of DNA methylation but were unable to visualize the dynamics of epigenetic regulation. MATERIALS &

resultsWe utilized a genomic DNA methylation reporter (GMR) system carrying Snrpn minimal promoter and CpG regions of

conclusionThe GMR reporter system can be useful for monitoring real-time epigenetic DNA modification at single-cell resolution.

Indexed as

Cell DifferentiationDNA MethylationEpigenesis, GeneticInduced Pluripotent Stem CellsMyocytes, CardiacAnimalsCDC2 Protein KinaseCpG IslandsGenes, ReporterMicePromoter Regions, GeneticSOXB1 Transcription FactorsCDC2 Protein KinaseSox2 protein, mouseSOXB1 Transcription FactorsCardiac differentiationcell cycleDNA methylationepigeneticsGMR (genomic DNA methylation reporter)iPSC (induced pluripotent stem cell)pyrosequencingsingle-cell imaging

Identifiers

PMID39601042
PMCPMC11622782

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.