Evidence map›Paper›PMID 39599815›Full record

ArticleViruses2024

Nano-Biomechanical Investigation of Phosphatidylserine-Mediated Ebola Viral Attachment via Human Gas6 and Axl.

Decheng Hou, Qian Mu, Weixuan Chen, Wenpeng Cao, Xiaohui Frank Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Decheng HouDepartment of Bioengineering, Lehigh University, Bethlehem, PA 18015, USA.ORCID 0000-0001-7936-1076
Qian MuDepartment of Biomedical Engineering, University of Massachusetts at Amherst, Amherst, MA 01003, USA.ORCID 0000-0003-4012-1862
Weixuan ChenDepartment of Biomedical Engineering, University of Massachusetts at Amherst, Amherst, MA 01003, USA.ORCID 0009-0003-2374-6681
Wenpeng CaoDepartment of Bioengineering, Lehigh University, Bethlehem, PA 18015, USA.
Xiaohui Frank ZhangDepartment of Bioengineering, Lehigh University, Bethlehem, PA 18015, USA.ORCID 0000-0002-8778-595X

Funding

Biomechanics of TIM Protein-Mediated Ebola Virus-Host Cell AdhesionR15AI133634 · NIAID · LEHIGH UNIVERSITY · PI JAGOTA, ANAND, ZHANG, XIAOHUI · 2018 to 2018
$435k
Biophysical characterization of SARS-CoV-2 spike protein - receptor interactionsR21AI163708 · NIAID · LEHIGH UNIVERSITY · PI IM, WONPIL, ZHANG, XIAOHUI · 2021 to 2022
$426k
National Science Foundation CBET-2226779NIAID NIH HHS R15 AI133634NIAID NIH HHS R21 AI163708NIH HHS AI133634NIH HHS AI163708
6 · The paper itself

Abstract

The Ebola virus is a deadly pathogen that has been threatening public health for decades. Recent studies have revealed alternative viral invasion routes where Ebola virus approaches cells via interactions among phosphatidylserine (PS), PS binding ligands such as Gas6, and TAM family receptors such as Axl. In this study, we investigate the interactions among phosphatidylserine on the Ebola viral-like particle (VLP) membrane, human Gas6, and human Axl using atomic force microscope-based single molecule force spectroscopy to compare their binding strength and affinity from a biomechanical perspective. The impact of calcium ions on their interactions is also studied and quantified to provide more details on the calcium-dependent phosphatidylserine-Gas6 binding mechanism. Our results indicate that, in the presence of calcium ions, the binding strengths of VLP-Gas6 and VLP-Gas6-Axl increase but are still weaker than that of Gas6-Axl, and the binding affinity of VLP-Gas6 and VLP-Gas6-Axl is largely improved. The binding strength and affinity of Gas6-Axl basically remain the same, indicating no impact in the presence of calcium ions. Together, our study suggests that, under physiological conditions with calcium present, the Ebola virus can utilize its membrane phosphatidylserine to dock on cell surface via Gas6-Axl bound complex.

Indexed as

Axl Receptor Tyrosine KinaseEbolavirusIntercellular Signaling Peptides and ProteinsPhosphatidylserinesReceptor Protein-Tyrosine KinasesVirus AttachmentCalciumGrowth Arrest-Specific Protein 6Hemorrhagic Fever, EbolaHumansMicroscopy, Atomic ForceProtein BindingProto-Oncogene ProteinsAXL protein, humanAxl Receptor Tyrosine KinaseCalciumGrowth Arrest-Specific Protein 6Intercellular Signaling Peptides and ProteinsPhosphatidylserinesProto-Oncogene ProteinsReceptor Protein-Tyrosine Kinasesatomic force microscopyAxlEbolaGas6single molecule force spectroscopyviral entry

Identifiers

PMID39599815
PMCPMC11599018

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.