Evidence map›Paper›PMID 39599534›Full record

ArticlePathogens (Basel, Switzerland)2024

Development and Validation of an Indirect and Blocking ELISA for the Serological Diagnosis of African Swine Fever.

Chukwunonso Onyilagha, Kaye Quizon, Dmytro Zhmendak, Ian El Kanoa, Thang Truong, Thanuja Ambagala, Alfonso Clavijo, Van Phan Le, Shawn Babiuk, Aruna Ambagala

Abstract readValidation Study
In one paragraph

Article in Pathogens (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chukwunonso OnyilaghaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.ORCID 0000-0002-5592-7095
Kaye QuizonNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3M4, Canada.
Dmytro ZhmendakNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
Ian El KanoaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
Thang TruongNational Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3M4, Canada.ORCID 0000-0002-0707-7452
Thanuja AmbagalaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
Alfonso ClavijoNational Bio and Agro-Defense Facility, Agricultural Research Service, United States Department of Agriculture, Manhattan, KS 66506, USA.
Van Phan LeDepartment of Microbiology and Infectious Disease, College of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 100000, Vietnam.ORCID 0000-0003-0583-4111
Shawn BabiukNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.ORCID 0000-0003-1265-7264
Aruna AmbagalaNational Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.ORCID 0000-0003-1783-2105

Funding

National Centre for Foreign Animal Disease, a department of the Canadian Food Inspection Agency, and partially supported by Swine Health Information Center Grant 20-085
6 · The paper itself

Abstract

African swine fever (ASF) is an economically devastating viral disease of pigs caused by the ASF virus (ASFV). The rapid global spread of ASF has increased the demand for ASF diagnostics to be readily available and accessible. No commercial ASF enzyme-linked immunosorbent assay (ELISA) kits are manufactured and licensed in North America. Here, we report the development of two serological diagnostic assays, a blocking ELISA (bELISA) based on ASFV glycoprotein p54 and an indirect ELISA (iELISA) based on ASFV glycoproteins p54 and p72. The assays showed high sensitivity and specificity and detected anti-ASFV antibodies in serum samples from experimentally infected animals as early as 8 days post-infection. The two assays were produced commercially (AsurDx

Indexed as

African Swine FeverAfrican Swine Fever VirusAntibodies, ViralEnzyme-Linked Immunosorbent AssaySensitivity and SpecificitySerologic TestsAnimalsAntigens, ViralSwineAntibodies, ViralAntigens, ViralAfrican swine feverblocking ELISAdiagnosticsindirect ELISAserology

Identifiers

PMID39599534
PMCPMC11597605

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.