Evidence map›Paper›PMID 39599494›Full record

ArticlePathogens (Basel, Switzerland)2024

DNA Barcoding Using 18S rRNA Gene Fragments for Identification of Tick-Borne Protists in Ticks in the Republic of Korea.

Badriah Alkathiri, Subin Lee, KyuSung Ahn, So Youn Youn, Mi-Sun Yoo, Hyang-Sim Lee, Yun Sang Cho, Jaeyun Jung, Kwangwon Seo, Soochong Kim and 5 more

Abstract read
In one paragraph

Article in Pathogens (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. First description ofInternational journal for parasitology. Parasites and wildlife · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Badriah AlkathiriCollege of Veterinary Medicine, Chungbuk National University, Chungbuk 28644, Republic of Korea.ORCID 0000-0001-9575-1007
Subin LeeCollege of Veterinary Medicine, Chungbuk National University, Chungbuk 28644, Republic of Korea.
KyuSung AhnBIOREEDS Research Institute, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea.
So Youn YounLaboratory of Parasitic and Honeybee Diseases, Bacterial Disease Division, Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.
Mi-Sun YooLaboratory of Parasitic and Honeybee Diseases, Bacterial Disease Division, Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.
Hyang-Sim LeeLaboratory of Parasitic and Honeybee Diseases, Bacterial Disease Division, Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.
Yun Sang ChoLaboratory of Parasitic and Honeybee Diseases, Bacterial Disease Division, Department of Animal & Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.ORCID 0000-0003-1346-7067
Jaeyun JungDepartment of Biochemistry, College of Medicine, Chungbuk National University, Chungbuk 28644, Republic of Korea.
Kwangwon SeoCollege of Veterinary Medicine, Chungbuk National University, Chungbuk 28644, Republic of Korea.ORCID 0000-0003-1620-4916
Soochong KimCollege of Veterinary Medicine, Chungbuk National University, Chungbuk 28644, Republic of Korea.ORCID 0000-0002-6739-9787
Rika Umemiya-ShirafujiNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.ORCID 0000-0001-7641-5526
Xuenan XuanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.ORCID 0000-0003-2780-110X
Dongmi KwakCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.ORCID 0000-0003-0876-3179
SungShik ShinBIOREEDS Research Institute, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea.
Seung-Hun LeeCollege of Veterinary Medicine, Chungbuk National University, Chungbuk 28644, Republic of Korea.ORCID 0000-0002-6244-0381

Funding

Animal and Plant Quarantine AgencyChungbuk National University BK21 program 2024
6 · The paper itself

Abstract

The objective of this study was to evaluate the diversity and prevalence of tick-borne protists in the Republic of Korea via DNA barcoding using 18S rRNA gene fragments and PCR. Between 2021 and 2022, questing ticks were collected using the flagging method, with a total of 13,375 ticks collected and pooled into 1003 samples. Of these, 50 tick pools were selected for DNA barcoding targeting the V4 and V9 regions of 18S rRNA using the MiSeq platform. A taxonomic analysis of the amplicon sequence variants identified three genera of protozoa, namely

Indexed as

DNA Barcoding, TaxonomicRNA, Ribosomal, 18SAnimalsDNA, ProtozoanIxodesPhylogenyRepublic of KoreaTheileriaTicksDNA, ProtozoanRNA, Ribosomal, 18Smetabarcodingnext-generation sequencingprotozoaticktick-borne pathogenvector

Identifiers

PMID39599494
PMCPMC11597262

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.