Evidence map›Paper›PMID 39596635›Full record

ArticleGenes2024

Comparison of TLR4 Genotype and TLR4 Pathway-Related Cytokines in Different Strains of Mice in Response to Pertussis Toxin Challenge.

Jie Wei, Lichan Wang, Chen Wei, Jiaona Guang, Hong Wang, Jiaqi Zhou, Huan Li, Xiao Ma, Bingfei Yue

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jie WeiDivision of Laboratory Animal Monitoring, National Institutes for Food and Drug Control, Beijing 102629, China.
Lichan WangDivision of Diphtheria, Tetanus and Pertussis Vaccine and Toxins, National Institutes for Food and Drug Control, Beijing 102629, China.
Chen WeiDivision of Diphtheria, Tetanus and Pertussis Vaccine and Toxins, National Institutes for Food and Drug Control, Beijing 102629, China.
Jiaona GuangDivision of Laboratory Animal Monitoring, National Institutes for Food and Drug Control, Beijing 102629, China.
Hong WangDivision of Laboratory Animal Monitoring, National Institutes for Food and Drug Control, Beijing 102629, China.
Jiaqi ZhouDivision of Laboratory Animal Monitoring, National Institutes for Food and Drug Control, Beijing 102629, China.ORCID 0009-0005-6182-1368
Huan LiDivision of Laboratory Animal Monitoring, National Institutes for Food and Drug Control, Beijing 102629, China.
Xiao MaDivision of Diphtheria, Tetanus and Pertussis Vaccine and Toxins, National Institutes for Food and Drug Control, Beijing 102629, China.
Bingfei YueDivision of Laboratory Animal Monitoring, National Institutes for Food and Drug Control, Beijing 102629, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe genetic background of Toll-like receptor 4 (TLR4) proved to be important in the induction of immune protection against

methodWe first determined the single-nucleotide polymorphisms (SNPs) in the TLR4 gene by using first-generation sequencing. Then, the cellular response, including the TLR4 mRNA expression and TLR4 signaling-related cytokines, of immune cells from different mouse strains after PT stimulation was determined.

resultThree missense mutation sites (rs13489092, rs13489093, rs13489097) of the TLR4 gene were found. ICR mice were homozygous without mutation, NIH mice were partially heterozygous, and BALB/c mice were homozygous with a missense mutation. The expression of TLR4 was repressed while the downstream cytokines were upregulated after PT stimulation differently among mouse strains. The IFN-β cytokine of the TRIF pathway was significantly increased in ICR mice (

conclusionsOur studies might provide useful referees to reduce the mouse-derived difference in the determination of vaccine titer and increase the comparability of the vaccine from different origins, as different mouse strains were used for vaccine development in different countries.

Indexed as

CytokinesMice, Inbred BALB CPertussis ToxinPolymorphism, Single NucleotideSignal TransductionToll-Like Receptor 4AnimalsFemaleGenotypeMiceMice, Inbred ICRMutation, MissenseWhooping CoughCytokinesPertussis ToxinTlr4 protein, mouseToll-Like Receptor 4cytokinesgenotypepertussis toxintoll-like receptor 4

Identifiers

PMID39596635
PMCPMC11593472

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.