ArticleInternational journal of molecular sciences2024
Site Matters: Differences in Gene Expression Profiles Along the Bovine Rumen Papilla During Subacute Rumen Acidosis.
Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Subacute rumen acidosis (SARA) is a significant concern in dairy cattle fed grain-rich diets. To elucidate the underlying pathophysiological mechanisms, ruminal papilla biopsies are often used. This study aimed to assess how the sampling site along the ruminal papilla influences gene expression profiles in rumen epithelium during SARA. Rumen biopsies from five ruminal-cannulated non-lactating Holstein cows were collected during feeding of a forage diet (FD) and seven (wk1) and 21 days (wk3) after transition to high-grain (HG) feeding. Gene expression in apical (AP), basal (BP), and total length (TP) papillae were compared using RT-qPCR. Significant diet-induced effects were observed in AP for
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