Evidence map›Paper›PMID 39594437›Full record

ArticleAntioxidants (Basel, Switzerland)2024

ZnO NPs Impair the Viability and Function of Porcine Granulosa Cells Through Autophagy Regulated by ROS Production.

Yifan Wang, Jing Lv, Guangyu Liu, Qichun Yao, Ziqi Wang, Ning Liu, Yutao He, Dmitry Il, Jakupov Isatay Tusupovich, Zhongliang Jiang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antioxidants (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yifan WangKey Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, College of Animal Science and Technology, Northwest Agriculture and Forestry University, Xianyang 712100, China.ORCID 0000-0001-6534-4531
Jing LvKey Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, College of Animal Science and Technology, Northwest Agriculture and Forestry University, Xianyang 712100, China.
Guangyu LiuKey Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, College of Animal Science and Technology, Northwest Agriculture and Forestry University, Xianyang 712100, China.ORCID 0000-0002-2479-3047
Qichun YaoAnimal Husbandry and Veterinary Station of Zhenba County, Hanzhong 723600, China.
Ziqi WangKey Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, College of Animal Science and Technology, Northwest Agriculture and Forestry University, Xianyang 712100, China.
Ning LiuKey Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, College of Animal Science and Technology, Northwest Agriculture and Forestry University, Xianyang 712100, China.ORCID 0000-0003-2948-8161
Yutao HeKey Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, College of Animal Science and Technology, Northwest Agriculture and Forestry University, Xianyang 712100, China.
Dmitry IlDepartment of Food Security, Agrotechnological Faculty, Kozybayev University, 86, Pushkin Street, Petropavlovsk 150000, Kazakhstan.
Jakupov Isatay TusupovichDepartment of Veterinary Medicine, Seifullin Kazakh Agro Technical Research University, 62, Zhenis Avenue, Astana 010011, Kazakhstan.ORCID 0000-0002-9373-2520
Zhongliang JiangKey Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, College of Animal Science and Technology, Northwest Agriculture and Forestry University, Xianyang 712100, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The zinc oxide nanoparticles (ZnO NPs) is one of the most extensively utilized metal oxide nanoparticles in biomedicine, human food, cosmetics and livestock farming. However, growing evidence suggests that there is a potential risk for humans and animals because of the accumulation of ZnO NPs in cells, which leads to cell death through several different pathways. Nevertheless, the effects of ZnO NPs on porcine granulosa cells (PGCs) and how ZnO NPs regulate the follicular cells are unknown. In this study, we aimed to elucidate the role of ZnO NPs in the porcine ovary by using PGCs. Firstly, we identified the characterization of ZnO NPs used in this study and the results showed that the size of ZnO NPs was 29.0 nm. The results also demonstrated that ZnO NPs impaired cell viability and decreased steroid hormone secretion in PGCs. In addition, ZnO NPs induced reactive oxygen species (ROS) production, leading to oxidative stress of PGCs. Meanwhile, ZnO NPs also triggered autophagy in PGCs by increasing the ratio of LC3-II/LC3-I, along with the expression of SQSTM1 and ATG7. Finally, the results from N-acetylcysteine (NAC) addition suggested that ZnO NPs promoted autophagy through the enhancement of ROS production. In summary, this study demonstrates that ZnO NPs impair the viability and function of PGCs through autophagy, which is regulated by ROS production.

Indexed as

autophagygranulosa cellporcineROSZnO NPs

Identifiers

PMID39594437
PMCPMC11591140

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.