ReviewFoods (Basel, Switzerland)2024
Methods for Detection, Extraction, Purification, and Characterization of Exopolysaccharides of Lactic Acid Bacteria-A Systematic Review.
Review in Foods (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Beyond Probiotics: Postbiotics for Healthy Aquaculture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Dietary Microbial Polysaccharides in Food Systems: Production, Functional Properties, and Applications.Food science & nutrition · 2026Review
- Endogenous CRISPR-Based Removal of Tetracycline Resistance in Bifidobacterium animalis subsp. lactis Through a Safe-by-Design Approach.Microbial biotechnology · 2026Article
- Emerging Microbiological and Sensor-Based Approaches for Biofilm Detection in Meat and Poultry Processing Environments.Foods (Basel, Switzerland) · 2026Review
- Fermentation-A Potential Game Changer in the Development of Plant-Based Cream Cheese Alternatives.Foods (Basel, Switzerland) · 2026Review
- Water-soluble sulfated exopolysaccharide from Cytobacillus oceanisediminis: Its production, characterization and biological activities.Folia microbiologica · 2026Article
- Postbiotics as Emerging Therapeutics for Allergic Diseases: A Novel Approach Beyond Live Biologics.Probiotics and antimicrobial proteins · 2026Review
- Enhanced extracellular polymeric substance production and structural remodeling under copper stress in Bacillus paralicheniformis: statistical optimization and characterization.World journal of microbiology & biotechnology · 2026Article
- A Fucose-containing Hetero-exopolysaccharide From Levilactobacillus brevis B151 Exhibits Potent Antimicrobial, Antibiofilm, and Antioxidant Activities.Probiotics and antimicrobial proteins · 2026Article
- Transcriptome profiling of a novelApplied and environmental microbiology · 2026Article
- Characterization of Mannose-Rich Exopolysaccharides from Kefir Lactic Acid Bacteria and Their Techno-Functional Potential in Fermented Milk.Foods (Basel, Switzerland) · 2026Article
- Valorization of Co-Products from Barbecue Sauce Production Through Fermentation Processes.Foods (Basel, Switzerland) · 2026Article
- Characterisation and mechanism of action of the antimicrobial activity of hetero-exopolysaccharide from Lentilactobacillus buchneri KU200793 against Staphylococcus aureus and Listeria monocytogenes.Archives of microbiology · 2026Article
- Endophytic bacteria and fungi as a source of polysaccharides: production and characterization techniques.Frontiers in microbiology · 2026Review
- High-throughput and genome-guided optimization of exopolysaccharide production in marine bacteria for sustainable biotechnology.Applied and environmental microbiology · 2025Article
- Study on the Enrichment of the Main Active Components inFoods (Basel, Switzerland) · 2025Article
- Lactic Acid Bacteria Exopolysaccharides Unveiling Multifaceted Insights from Structure to Application in Foods and Health Promotion.Foods (Basel, Switzerland) · 2025Review
- motA-mediated flagellar motility modulates biofilm formation and competitive nodulation inFrontiers in microbiology · 2025Article
- Anticorrosion potential of a bioemulsifier produced byFrontiers in microbiology · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Exopolysaccharides (EPSs) are large-molecular-weight, complex carbohydrate molecules and extracellularly secreted bio-polymers released by many microorganisms, including lactic acid bacteria (LAB). LAB are well known for their ability to produce a wide range of EPSs, which has received major attention. LAB-EPSs have the potential to improve health, and their applications are in the food and pharmaceutical industries. Several methods have been developed and optimized in recent years for producing, extracting, purifying, and characterizing LAB-produced EPSs. The simplest method of evaluating the production of EPSs is to observe morphological features, such as ropy and mucoid appearances of colonies. Ethanol precipitation is widely used to extract the EPSs from the cell-free supernatant and is generally purified using dialysis. The most commonly used method to quantify the carbohydrate content is phenol-sulfuric acid. The structural characteristics of EPSs are identified via Fourier transform infrared, nuclear magnetic resonance, and X-ray diffraction spectroscopy. The molecular weight and composition of monosaccharides are determined through size-exclusion chromatography, thin-layer chromatography, gas chromatography, and high-performance liquid chromatography. The surface morphology of EPSs is observed via scanning electron microscopy and atomic force microscopy, whereas thermal characteristics are determined through thermogravimetry analysis, derivative thermogravimetry, and differential scanning calorimetry. In the present review, we discuss the different existing methods used for the detailed study of LAB-produced EPSs, which provide a comprehensive guide on LAB-EPS preparation, critically evaluating methods, addressing knowledge gaps and key challenges, and offering solutions to enhance reproducibility, scalability, and support for both research and industrial applications.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.