ArticleFEBS letters2025
Thermodynamic versus kinetic basis for the high conformational stability of nanobodies for therapeutic applications.
Article in FEBS letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Impact of Ser81 phosphorylation on alanine: glyoxylate aminotransferase associated with Primary hyperoxaluria type I.Molecular biomedicine · 2026Article
- YMN-V115: a novel humanized BCMA/GPRC5D/CD3 trispecific antibody in relapsed/refractory multiple myeloma.Journal for immunotherapy of cancer · 2026Article
- NbBayesLM: bayesian prediction of nanobody thermostability using protein language model.Frontiers in bioinformatics · 2026Article
- Article
- Magnetic Nanoworm-Based Screening of Yeast Library for Anti-PEG Nanobodies.Molecular pharmaceutics · 2025Article
- Expression of cellulase in Candida glycerogenes and strengthen the expression level for application in residue-containing fermentation to enhance glycerol production.Biotechnology letters · 2025Review
- A Simple Analysis of the Second (Extra) Disulfide Bridge of VMolecules (Basel, Switzerland) · 2024Article
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Authors and funding
4 authors.
Funding
Abstract
Nanobodies (NB) are powerful tools for biotechnological and therapeutic applications. They strongly bind to their targets and are very stable. Early studies showed that NB unfolding is reversible and can be analyzed by equilibrium thermodynamics, whereas more recent studies focused on their kinetic stability in very harsh conditions that are far from storage or physiological temperatures (4-37 °C). Here, we show that the thermodynamic view of NB stability holds in a wide range of temperatures (18-100 °C). The thermodynamic stability of three different NBs did not correlate with binding affinity for their target. Alpha-Fold 2 analyses of these NBs showed structural differences in the binding site and hydrogen bond networks. We expect that our approach will be helpful to improve our capacity to enhance structure-function-stability relationships of NB.
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