ArticleNature chemistry2025
Unmasking the reverse catalytic activity of 'ene'-reductases for asymmetric carbonyl desaturation.
Article in Nature chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Redirecting a Native Ene-Reductase Toward Desaturation With Reverse Enantioselectivity.Angewandte Chemie (International ed. in English) · 2026Article
- Article
- Biocatalytic site- and stereoselective carbonyl desaturation for late-stage functionalization of cyclic ketones.Nature chemistry · 2026Article
- Mining and engineering of ene-reductases from marine sediment metagenome for prochiral ACE inhibitor synthesis.Applied and environmental microbiology · 2026Article
- Stereoselective cyano translocation reaction enabled by photoenzymatic catalysis.Nature communications · 2026Article
- Dynamic Enantioconvergent Desaturation of 4,5-Disubstituted γ-Lactones in Whole Cells ofJournal of the American Chemical Society · 2026Article
- Bottleable triplet nitrenes.Nature chemistry · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
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Abstract
Carbonyl desaturation is a fundamental reaction widely practised in organic synthesis. While numerous methods have been developed to expand the scope of this important transformation, most of them necessitate multi-step protocols or suffer from the use of high loadings of metal or strong oxidizing conditions. Moreover, approaches that can achieve precise stereochemical control of the desaturation process are extremely rare. Here we report a biocatalytic platform for desymmetrizing desaturation of cyclohexanones to generate diverse cyclohexenones bearing a remote quaternary stereogenic centre, by reengineering 'ene'-reductases to efficiently mediate dehydrogenation, the reverse process of their native activity. This 'ene'-reductase-based desaturation system operates under mild conditions with air as the terminal oxidant, tolerates oxidation-sensitive or metal-incompatible functional groups and, more importantly, exhibits unparalleled stereoselectivity compared with those achieved with small-molecule catalysts. Mechanistic investigations suggest that the reaction proceeded through α-deprotonation followed by a rate-determining β-hydride transfer.
Identifiers
39592841What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.