Evidence map›Paper›PMID 39592494›Full record

ArticleClinical oral investigations2024

Possible immunomodulatory role of Filifactor alocis through beta-defensin 2 in gingival keratinocytes.

Lorena S Gutierrez, Daniela L Zandim-Barcelos, Sigrun Eick, Maria Eduarda S Lopes, Joni A Cirelli, Andressa V B Nogueira, James Deschner

Abstract read
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Article in Clinical oral investigations, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lorena S GutierrezDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University-UNESP, Araraquara, SP, 14801-903, Brazil.
Daniela L Zandim-BarcelosDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University-UNESP, Araraquara, SP, 14801-903, Brazil. daniela.zandim-barcelos@unesp.br.ORCID 0000-0001-5442-7868
Sigrun EickLaboratory of Oral Microbiology, Department of Periodontology, University of Bern, Bern, 3010, Switzerland.ORCID 0000-0002-4619-2461
Maria Eduarda S LopesDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University-UNESP, Araraquara, SP, 14801-903, Brazil.ORCID 0000-0003-4878-3174
Joni A CirelliDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University-UNESP, Araraquara, SP, 14801-903, Brazil.ORCID 0000-0002-7082-9290
Andressa V B NogueiraDepartment of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, 55131, Mainz, Germany.ORCID 0000-0002-2756-5947
James DeschnerDepartment of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, 55131, Mainz, Germany.ORCID 0000-0002-8808-8769

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88881.144012/2017-01Deutscher Akademischer Austauschdienst 57391253
6 · The paper itself

Abstract

objectivesThe present study aimed to investigate a possible immunomodulatory role of the periodontopathogen Filifactor alocis through the antimicrobial peptide hBD-2 on the expression of chemokines in human gingival keratinocytes. MATERIALS AND

methodsCells were cultured in the presence or absence of periodontopathogenic bacteria, such as F. alocis, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola, to evaluate the regulation of hBD-2, CXCL8 and CCL20. Furthermore, the cells were exposed or not to hBD-2 and the expression of CXCL8 and CCL20 and their receptors was evaluated.

resultsAll bacteria induced a significant upregulation of hBD-2, CXCL8, and CCL20 gene expressions. In addition, F. alocis significantly increased their protein levels, as detected by ELISA. Pre-incubation of the cells with the TLR2 inhibitor resulted in a significant downregulation of hBD-2 expression in F. alocis-treated cells. Gingival keratinocytes exposed to hBD-2 resulted in a significant and dose-dependent increase of all chemokines and their receptors.

conclusionsF. alocis increased the production of chemotactic cytokines, suggesting an increase in the recruitment of immunoinflammatory cells in periodontal diseases. The chemotaxis-promoting effect is partly direct, but is also mediated via hBD-2. F. alocis stimulates the synthesis of hBD-2, which in turn could promote the expression and synthesis of these chemokines and their receptors. In addition, hBD-2 has an autostimulatory effect and stimulates the synthesis of these chemokines, so that the chemotaxis triggered by F. alocis is further fueled. CLINICAL RELEVANCE: F. alocis and hBD-2 have a significant role in periodontitis, showing their importance for diagnostic and treatment approaches.

Indexed as

beta-DefensinsChemokine CCL20Enzyme-Linked Immunosorbent AssayGingivaKeratinocytesPorphyromonas gingivalisAggregatibacter actinomycetemcomitansCells, CulturedEubacterialesGene ExpressionHumansInterleukin-8Treponema denticolaUp-Regulationbeta-DefensinsCCL20 protein, humanChemokine CCL20DEFB4A protein, humanInterleukin-8Beta-defensinsEpithelial cellsImmune responseInflammationPeriodontitis

Identifiers

PMID39592494

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.