Evidence map›Paper›PMID 39591421›Full record

ArticlePloS one2024

Exploring the feasibility of using mice as a substitute model for investigating microglia in aging and Alzheimer's disease though single cell analysis.

Rong He, Qiang Zhang, Limei Wang, Yiwen Hu, Yue Qiu, Jia Liu, Dingyun You, Jishuai Cheng, Xue Cao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Rong HeLaboratory Animal Department, Kunming Medical University, Kunming, Yunnan, China.ORCID 0000-0002-3850-5704
Qiang ZhangLaboratory Animal Department, Kunming Medical University, Kunming, Yunnan, China.
Limei WangLaboratory Animal Department, Kunming Medical University, Kunming, Yunnan, China.
Yiwen HuLaboratory Animal Department, Kunming Medical University, Kunming, Yunnan, China.
Yue QiuDermatology Department of Xiangya Hospital, Central South University, Changsha, Hunan, China.
Jia LiuLaboratory Animal Department, Kunming Medical University, Kunming, Yunnan, China.
Dingyun YouSchool of Public Health, Kunming Medical University, Kunming, Yunnan, China.
Jishuai ChengLaboratory Animal Department, Kunming Medical University, Kunming, Yunnan, China.
Xue CaoLaboratory Animal Department, Kunming Medical University, Kunming, Yunnan, China.ORCID 0000-0003-3775-8889

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo guide animal experiments, we investigated the similarities and differences between humans and mice in aging and Alzheimer's disease (AD) at the single-nucleus RNA sequencing (snRNA-seq) or single-cell RNA sequencing (scRNA-seq) level.

methodsMicroglia cells were extracted from dataset GSE198323 of human post-mortem hippocampus. The distributions and proportions of microglia subpopulation cell numbers related to AD or age were compared. This comparison was done between GSE198323 for humans and GSE127892 for mice, respectively. The Seurat R package and harmony R package were used for data analysis and batch effect correction. Differentially expressed genes (DEGs) were identified by FindMarkers function with MAST test. Comparative analyses were conducted on shared genes in DEGs associated with age and AD. The analyses were done between human and mouse using various bioinformatics techniques. The analysis of genes in DEGs related to age was conducted. Similarly, the analysis of genes in DEGs related to AD was performed. Cross-species analyses were conducted using orthologous genes. Comparative analyses of pseudotime between humans and mice were performed using Monocle2.

results(1) Similarities: The proportion of microglial subpopulation Cell_APOE/Apoe shows consistent trends, whether in AD or normal control (NC) groups in both humans and mice. The proportion of Cell_CX3CR1/Cx3cr1, representing homeostatic microglia, remains stable with age in NC groups across species. Tuberculosis and Fc gamma R-mediated phagocytosis pathways are shared in microglia responses to age and AD across species, respectively. (2) Differences: IL1RAPL1 and SPP1 as marker genes are more identifiable in human microglia compared to their mouse counterparts. Most genes of DEGs associated with age or AD exhibit different trends between humans and mice. Pseudotime analyses demonstrate varying cell density trends in microglial subpopulations, depending on age or AD across species.

conclusionsMouse Apoe and Cell_Apoe maybe serve as proxies for studying human AD, while Cx3cr1 and Cell_Cx3cr1 are suitable for human aging studies. However, AD mouse models (App_NL_G_F) have limitations in studying human genes like IL1RAPL1 and SPP1 related to AD. Thus, mouse models cannot fully replace human samples for AD and aging research.

Indexed as

AgingAlzheimer DiseaseDisease Models, AnimalMicrogliaSingle-Cell AnalysisAnimalsCX3C Chemokine Receptor 1Feasibility StudiesHippocampusHumansMaleMiceCX3C Chemokine Receptor 1

Identifiers

PMID39591421
PMCPMC11594518

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.